التفاصيل البيبلوغرافية
| العنوان: |
Intracellular autofluorescence: a biomarker for epithelial cancer stem cells. |
| المؤلفون: |
Miranda-Lorenzo, Irene, Dorado, Jorge, Lonardo, Enza, Alcala, Sonia, Serrano, Alicia G, Clausell-Tormos, Jenifer, Cioffi, Michele, Megias, Diego, Zagorac, Sladjana, Balic, Anamaria, Hidalgo, Manuel, Erkan, Mert, Kleeff, Joerg, Scarpa, Aldo, Sainz, Bruno, Heeschen, Christopher |
| المصدر: |
Nature Methods; Nov2014, Vol. 11 Issue 11, p1161-1169, 9p, 6 Graphs |
| مصطلحات موضوعية: |
CANCER stem cells, CANCER cells, METASTASIS, PHENOTYPES, ENTEROTYPES |
| مستخلص: |
Cancer stem cells (CSCs) are thought to drive tumor growth, metastasis and chemoresistance. Although surface markers such as CD133 and CD44 have been successfully used to isolate CSCs, their expression is not exclusively linked to the CSC phenotype and is prone to environmental alteration. We identified cells with an autofluorescent subcellular compartment that exclusively showed CSC features across different human tumor types. Primary tumor-derived autofluorescent cells did not overlap with side-population (SP) cells, were enriched in sphere culture and during chemotherapy, strongly expressed pluripotency-associated genes, were highly metastatic and showed long-term in vivo tumorigenicity, even at the single-cell level. Autofluorescence was due to riboflavin accumulation in membrane-bounded cytoplasmic structures bearing ATP-dependent ABCG2 transporters. In summary, we identified and characterized an intrinsic autofluorescent phenotype in CSCs of diverse epithelial cancers and used this marker to isolate and characterize these cells. [ABSTRACT FROM AUTHOR] |
|
Copyright of Nature Methods is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
