Perforin pores in the endosomal membrane trigger release of endocytosed granzyme B to the cytosol of target cells
| العنوان: | Perforin pores in the endosomal membrane trigger release of endocytosed granzyme B to the cytosol of target cells |
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| المؤلفون: | Ing Swie Goping, Jerome Thiery, Judy Lieberman, Emmanuel Boucrot, Michael Walch, Denis Martinvalet, R. Chris Bleackley, Dennis Keefe, Steeve Boulant, Tomas Kirchhausen |
| المصدر: | Nature immunology |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Cell, Granzymes, Cell membrane, 0302 clinical medicine, Cytosol, Immunology and Allergy, Killer Cells, Ammonium Chloride, Animals, Apoptosis, Cell Membrane, Endocytosis, Endosomes, Flow Cytometry, HeLa Cells, Humans, Killer Cells, Natural, Macrolides, Microscopy, Confocal, Microscopy, Video, Pore Forming Cytotoxic Proteins, Rats, Immunology, perforin, 0303 health sciences, Microscopy, biology, apoptosis, Video, hemic and immune systems, Research Highlight, Cell biology, medicine.anatomical_structure, Confocal, Natural, Endosome, chemical and pharmacologic phenomena, Article, 03 medical and health sciences, medicine, NK cell, 030304 developmental biology, granzyme, Granzyme B, Perforin, Granzyme, biology.protein, 030215 immunology, cytotoxic T cell |
| الوصف: | How the pore-forming protein perforin delivers apoptosis-inducing granzymes to the cytosol of target cells is uncertain. Perforin induces a transient Ca2+ flux in the target cell, which triggers a process to repair the damaged cell membrane. As a consequence, both perforin and granzymes are endocytosed into enlarged endosomes called 'gigantosomes'. Here we show that perforin formed pores in the gigantosome membrane, allowing endosomal cargo, including granzymes, to be gradually released. After about 15 min, gigantosomes ruptured, releasing their remaining content. Thus, perforin delivers granzymes by a two-step process that involves first transient pores in the cell membrane that trigger the endocytosis of granzyme and perforin and then pore formation in endosomes to trigger cytosolic release. |
| اللغة: | English |
| تدمد: | 1529-2916 1529-2908 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58b26a59f7ceacd9cc1418844b232b80Test http://europepmc.org/articles/PMC3140544Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....58b26a59f7ceacd9cc1418844b232b80 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15292916 15292908 |
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