دورية
The BAF chromatin remodeler synergizes with RNA polymerase II and transcription factors to evict nucleosomes
| العنوان: | The BAF chromatin remodeler synergizes with RNA polymerase II and transcription factors to evict nucleosomes |
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| المؤلفون: | Brahma, Sandipan, Henikoff, Steven |
| المصدر: | Nature Genetics; 20230101, Issue: Preprints p1-12, 12p |
| مستخلص: | Chromatin accessibility is a hallmark of active transcription and entails ATP-dependent nucleosome remodeling, which is carried out by complexes such as Brahma-associated factor (BAF). However, the mechanistic links between transcription, nucleosome remodeling and chromatin accessibility are unclear. Here, we used a chemical–genetic approach coupled with time-resolved chromatin profiling to dissect the interplay between RNA Polymerase II (RNAPII), BAF and DNA-sequence-specific transcription factors in mouse embryonic stem cells. We show that BAF dynamically unwraps and evicts nucleosomes at accessible chromatin regions, while RNAPII promoter-proximal pausing stabilizes BAF chromatin occupancy and enhances ATP-dependent nucleosome eviction by BAF. We find that although RNAPII and BAF dynamically probe both transcriptionally active and Polycomb-repressed genomic regions, pluripotency transcription factor chromatin binding confers locus specificity for productive chromatin remodeling and nucleosome eviction by BAF. Our study suggests a paradigm for how functional synergy between dynamically acting chromatin factors regulates locus-specific nucleosome organization and chromatin accessibility. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 10614036 15461718 |
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| DOI: | 10.1038/s41588-023-01603-8 |