التفاصيل البيبلوغرافية
| العنوان: |
A damaged genome's transcriptional landscape through multilayered expression profiling around in situ-mapped DNA double-strand breaks. |
| المؤلفون: |
Iannelli, Fabio, Galbiati, Alessandro, Capozzo, Ilaria, Nguyen, Quan, Magnuson, Brian, Michelini, Flavia, D'Alessandro, Giuseppina, Cabrini, Matteo, Roncador, Marco, Francia, Sofia, Crosetto, Nicola, Ljungman, Mats, Carninci, Piero, d'Adda di Fagagna, Fabrizio |
| المصدر: |
Nature Communications; May2017, Vol. 8 Issue 5, p15656, 1p |
| مستخلص: |
Of the many types of DNA damage, DNA double-strand breaks (DSBs) are probably the most deleterious. Mounting evidence points to an intricate relationship between DSBs and transcription. A cell system in which the impact on transcription can be investigated at precisely mapped genomic DSBs is essential to study this relationship. Here in a human cell line, we map genome-wide and at high resolution the DSBs induced by a restriction enzyme, and we characterize their impact on gene expression by four independent approaches by monitoring steady-state RNA levels, rates of RNA synthesis, transcription initiation and RNA polymerase II elongation. We consistently observe transcriptional repression in proximity to DSBs. Downregulation of transcription depends on ATM kinase activity and on the distance from the DSB. Our study couples for the first time, to the best of our knowledge, high-resolution mapping of DSBs with multilayered transcriptomics to dissect the events shaping gene expression after DSB induction at multiple endogenous sites. [ABSTRACT FROM AUTHOR] |
|
Copyright of Nature Communications is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder