Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-α
| العنوان: | Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-α |
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| المؤلفون: | Yu-Chung Pien, Cédric Grauffel, Ching-Jou Chen, Su-Yi Tsai, Carmay Lim, Kuo-Chiang Hsia, Chih-Chia Chang |
| المصدر: | Nature Communications, Vol 10, Iss 1, Pp 1-16 (2019) Nature Communications |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | alpha Karyopherins, 0301 basic medicine, Vesicle fusion, Science, Vesicular Transport Proteins, Golgi Apparatus, Mitosis, General Physics and Astronomy, Importin, Crystallography, X-Ray, Autoantigens, Membrane Fusion, environment and public health, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, symbols.namesake, 0302 clinical medicine, Golgi, Humans, Transport receptors, Phosphorylation, lcsh:Science, Metaphase, X-ray crystallography, Multidisciplinary, Chemistry, Golgi Matrix Proteins, Membrane Proteins, Lipid bilayer fusion, General Chemistry, Golgi apparatus, beta Karyopherins, Cell biology, G2 Phase Cell Cycle Checkpoints, HEK293 Cells, ran GTP-Binding Protein, 030104 developmental biology, Golgi disassembly, Ran, symbols, lcsh:Q, Nuclear transport, 030217 neurology & neurosurgery, Protein Binding |
| الوصف: | To facilitate proper mitotic cell partitioning, the Golgi disassembles by suppressing vesicle fusion. However, the underlying mechanism has not been characterized previously. Here, we report a Ran pathway-independent attenuation mechanism that allows Importin-α (a nuclear transport factor) to suppress the vesicle fusion mediated by p115 (a vesicular tethering factor) and is required for mitotic Golgi disassembly. We demonstrate that Importin-α directly competes with p115 for interaction with the Golgi protein GM130. This interaction, promoted by a phosphate moiety on GM130, is independent of Importin-β and Ran. A GM130 K34A mutant, in which the Importin-α-GM130 interaction is specifically disrupted, exhibited abundant Golgi puncta during metaphase. Importantly, a mutant showing enhanced p115-GM130 interaction presented proliferative defects and G2/M arrest, demonstrating that Importin-α-GM130 binding modulates the Golgi disassembly that governs mitotic progression. Our findings illuminate that the Ran and kinase-phosphatase pathways regulate multiple aspects of mitosis coordinated by Importin-α (e.g. spindle assembly, Golgi disassembly). Golgi disassembly is required for mitosis and occurs by vesicle fusion suppression, although the mechanism is unclear. Here, Chang et al. show, with quantitative analyses and crystallography, that Importin-α regulates this process by blocking GM130-p115 interactions in a Ran pathway-independent way. |
| تدمد: | 2041-1723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::268d4f9d6aaee28112c3fca0cacac14fTest https://doi.org/10.1038/s41467-019-12207-4Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....268d4f9d6aaee28112c3fca0cacac14f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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