Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole
| العنوان: | Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole |
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| المؤلفون: | Yiling Hu, Li Jiang, Feifei Wang, Yanhong Liu, Ankanahalli N. Nanjaraj Urs, Dazhi Liu, Xuyang Liu, Yan Zhou, Huimin Zhao, Ee Lui Ang, Yan Zhang, Suwen Zhao, Yifeng Wei, Jinyu Yin |
| المصدر: | Nature Communications, Vol 9, Iss 1, Pp 1-8 (2018) Nature Communications |
| بيانات النشر: | Nature Publishing Group, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, endocrine system, Carboxy-Lyases, Decarboxylation, Science, Glycine, General Physics and Astronomy, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, chemistry.chemical_compound, Malodorant, Amino Acid Sequence, lcsh:Science, Conserved Sequence, Phylogeny, chemistry.chemical_classification, Multidisciplinary, Indoleacetic Acids, 030102 biochemistry & molecular biology, biology, urogenital system, Tryptophan, Genomics, General Chemistry, biology.organism_classification, Recombinant Proteins, Skatole, Actinobacteria, 030104 developmental biology, Enzyme, chemistry, Biochemistry, Fermentation, Odorants, Biocatalysis, lcsh:Q, Anaerobic bacteria, Bacteria |
| الوصف: | Skatole is a malodorous compound that contributes to the characteristic smell of animal faeces. Although skatole has long been known to originate from bacterial tryptophan fermentation, the enzyme catalysing its formation has so far remained elusive. Here we report the use of comparative genomics for the discovery of indoleacetate decarboxylase, an O2-sensitive glycyl radical enzyme catalysing the decarboxylation of indoleacetate to form skatole as the terminal step of tryptophan fermentation in certain anaerobic bacteria. We describe its biochemical characterization and compare it to other glycyl radical decarboxylases. Indoleacetate decarboxylase may serve as a genetic marker for the identification of skatole-producing environmental and human-associated bacteria, with impacts on human health and the livestock industry. Skatole is a bacterial metabolite responsible for boar taint and the objectionable smell of manure. Here, the authors elucidate the final step of skatole biosynthesis, describing the discovery and biochemical characterization of the enzyme catalysing the conversion of indoleacetate into skatole. |
| اللغة: | English |
| تدمد: | 2041-1723 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78bdf4e6adb7893fa57f6b197a57c257Test http://link.springer.com/article/10.1038/s41467-018-06627-xTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....78bdf4e6adb7893fa57f6b197a57c257 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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