دورية
Synthetic O-acetylated sialosides facilitate functional receptor identification for human respiratory viruses
| العنوان: | Synthetic O-acetylated sialosides facilitate functional receptor identification for human respiratory viruses |
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| المؤلفون: | Li, Zeshi, Lang, Yifei, Liu, Lin, Bunyatov, Mehman I., Sarmiento, Angelic Isaza, de Groot, Raoul J., Boons, Geert-Jan |
| المصدر: | Nature Chemistry; 20210101, Issue: Preprints p1-8, 8p |
| مستخلص: | The transmission of viruses from animal reservoirs to humans poses major threats to public health. Preparedness for future zoonotic outbreaks requires a fundamental understanding of how viruses of animal origin have adapted to binding to a cell surface component and/or receptor of the new host. Here we report on the specificities of human and animal viruses that engage with O-acetylated sialic acid, which include betacoronaviruses, toroviruses and influenza C and D viruses. Key to these studies was the development of a chemoenzymatic methodology that can provide almost any sialate-acetylation pattern. A collection of O-acetylated sialoglycans was printed as a microarray for the determination of receptor specificity. These studies showed host-specific patterns of receptor recognition and revealed that three distinct human respiratory viruses uniquely bind 9-O-acetylated α2,8-linked disialoside. Immunofluorescence and cell entry studies support that such a glycotope as part of a ganglioside is a functional receptor for human coronaviruses. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 17554330 17554349 |
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| DOI: | 10.1038/s41557-021-00655-9 |