Ligand-induced monoubiquitination of BIK1 regulates plant immunity
| العنوان: | Ligand-induced monoubiquitination of BIK1 regulates plant immunity |
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| المؤلفون: | Jinggeng Zhou, Eugenia Russinova, Lucas Alves Neubus Claus, Junmin Peng, Michelle E. Leslie, Antje Heese, Xiao Yu, Zhiping Wu, Ping He, Bo Li, Kai Tao, Brett M. Tyler, Daniel Valentin Savatin, Jun Liu, Libo Shan, Xiyu Ma |
| المصدر: | NATURE |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, CYTOPLASMIC KINASES, Ubiquitylation, DEFENSE, Endosome, Endocytic cycle, RECOGNITION RECEPTOR FLS2, Kinases, UBIQUITINATION, 01 natural sciences, 03 medical and health sciences, Ubiquitin, Monoubiquitination, Protein phosphorylation, INDUCED ENDOCYTOSIS, Pattern recognition receptors in plants, COMPLEX, Multidisciplinary, biology, Kinase, Chemistry, fungi, Pattern recognition receptor, Biology and Life Sciences, DEGRADATION, Cell biology, 030104 developmental biology, Plant signalling, NADPH OXIDASE RBOHD, biology.protein, Phosphorylation, 010606 plant biology & botany |
| الوصف: | The detection of microorganism-associated ligands by plant cells activates a signalling cascade in which the kinase BIK1 is monoubiquinated, released from the FLS2-BAK1 complex, and internalized by endocytosis. Recognition of microbe-associated molecular patterns (MAMPs) by pattern recognition receptors (PRRs) triggers the first line of inducible defence against invading pathogens(1-3). Receptor-like cytoplasmic kinases (RLCKs) are convergent regulators that associate with multiple PRRs in plants(4). The mechanisms that underlie the activation of RLCKs are unclear. Here we show that when MAMPs are detected, the RLCK BOTRYTIS-INDUCED KINASE 1 (BIK1) is monoubiquitinated following phosphorylation, then released from the flagellin receptor FLAGELLIN SENSING 2 (FLS2)-BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) complex, and internalized dynamically into endocytic compartments. The Arabidopsis E3 ubiquitin ligases RING-H2 FINGER A3A (RHA3A) and RHA3B mediate the monoubiquitination of BIK1, which is essential for the subsequent release of BIK1 from the FLS2-BAK1 complex and activation of immune signalling. Ligand-induced monoubiquitination and endosomal puncta of BIK1 exhibit spatial and temporal dynamics that are distinct from those of the PRR FLS2. Our study reveals the intertwined regulation of PRR-RLCK complex activation by protein phosphorylation and ubiquitination, and shows that ligand-induced monoubiquitination contributes to the release of BIK1 family RLCKs from the PRR complex and activation of PRR signalling. |
| وصف الملف: | application/pdf |
| تدمد: | 1476-4687 0028-0836 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b077aa45a8b7ce1bb050476930e78bc8Test https://doi.org/10.1038/s41586-020-2210-3Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b077aa45a8b7ce1bb050476930e78bc8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14764687 00280836 |
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