Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex
| العنوان: | Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex |
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| المؤلفون: | Alexandros Poulopoulos, Alexander James Murphy, Rory Kirchner, Abdulkadir Ozkan, Patrick Davis, Jeffrey D. Macklis, John Hatch |
| المصدر: | Nature. 565:356-360 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Nervous system, Proteome, Growth Cones, Cell Growth Process, Cell Growth Processes, Cell Separation, Biology, Article, Mice, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, Biological neural network, medicine, Animals, Axon, Growth cone, Cerebral Cortex, Multidisciplinary, TOR Serine-Threonine Kinases, Axons, 030104 developmental biology, medicine.anatomical_structure, Cerebral cortex, Female, Neuron, Transcriptome, Neuroscience, 030217 neurology & neurosurgery |
| الوصف: | The development of neural circuits relies on axon projections establishing diverse, yet well-defined, connections between areas of the nervous system. Each projection is formed by growth cones-subcellular specializations at the tips of growing axons, encompassing sets of molecules that control projection-specific growth, guidance, and target selection1. To investigate the set of molecules within native growth cones that form specific connections, here we developed growth cone sorting and subcellular RNA-proteome mapping, an approach that identifies and quantifies local transcriptomes and proteomes from labelled growth cones of single projections in vivo. Using this approach on the developing callosal projection of the mouse cerebral cortex, we mapped molecular enrichments in trans-hemispheric growth cones relative to their parent cell bodies, producing paired subcellular proteomes and transcriptomes from single neuron subtypes directly from the brain. These data provide generalizable proof-of-principle for this approach, and reveal molecular specializations of the growth cone, including accumulations of the growth-regulating kinase mTOR2, together with mRNAs that contain mTOR-dependent motifs3,4. These findings illuminate the relationships between subcellular distributions of RNA and protein in developing projection neurons, and provide a systems-level approach for the discovery of subtype- and stage-specific molecular substrates of circuit wiring, miswiring, and the potential for regeneration. |
| تدمد: | 1476-4687 0028-0836 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88141a3e97ee0eb3710e041d355ec9dcTest https://doi.org/10.1038/s41586-018-0847-yTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....88141a3e97ee0eb3710e041d355ec9dc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14764687 00280836 |
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