Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes
| العنوان: | Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes |
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| المؤلفون: | Hazel Lin, Cristina Ponti, Arianna Gazzi, Amaya Ortega, Amaia Zurutuza, Alberto Bianco, Ester Vázquez, Aurelia Tubaro, Silvio Sosa, Marco Pelin, Maurizio Prato |
| المساهمون: | Pelin, Marco, Lin, Hazel, Gazzi, Arianna, Sosa, Silvio, Ponti, Cristina, Ortega, Amaya, Zurutuza, Amaia, Vázquez, Ester, Prato, Maurizio, Tubaro, Aurelia, Bianco, Alberto, Immunopathologie et chimie thérapeutique (ICT), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Nanomaterials Volume 10 Issue 8 Nanomaterials, MDPI, 2020, 10 (8), pp.1602. ⟨10.3390/nano10081602⟩ Nanomaterials, Vol 10, Iss 1602, p 1602 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | autophagy, Biocompatibility, General Chemical Engineering, media_common.quotation_subject, 2D material, 02 engineering and technology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 010402 general chemistry, medicine.disease_cause, 01 natural sciences, Article, lcsh:Chemistry, biocompatibility, medicine, Cytotoxic T cell, [CHIM]Chemical Sciences, General Materials Science, Viability assay, Internalization, Cytotoxicity, media_common, carbon nanomaterials, Chemistry, carbon nanomaterial, Autophagy, 021001 nanoscience & nanotechnology, 2D materials, dermal toxicity, 0104 chemical sciences, nervous system diseases, HaCaT, lcsh:QD1-999, Biophysics, 0210 nano-technology, Oxidative stress |
| الوصف: | International audience; In the frame of graphene-based material (GBM) hazard characterization, particular attention should be given to the cutaneous effects. Hence, this study investigates if HaCaT skin keratinocytes exposed to high concentrations of few-layer graphene (FLG) or partially dehydrated graphene oxide (d-GO) for a short time can recover from the cytotoxic insult, measured by means of cell viability, mitochondrial damage and oxidative stress, after GBM removal from the cell medium. When compared to 24 or 72 h continuous exposure, recovery experiments suggest that the cytotoxicity induced by 24 h exposure to GBM is only partially recovered after 48 h culture in GBM-free medium. This partial recovery, higher for FLG as compared to GO, is not mediated by autophagy and could be the consequence of GBM internalization into cells. The ability of GBMs to be internalized inside keratinocytes together with the partial reversibility of the cellular damage is important in assessing the risk associated with skin exposure to GBM-containing devices. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2079-4991 |
| DOI: | 10.3390/nano10081602 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ea73cabe42b43614b7f1131bde51b29Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4ea73cabe42b43614b7f1131bde51b29 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20794991 |
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| DOI: | 10.3390/nano10081602 |