High-Contrast Imaging of Nanodiamonds in Cells by Energy Filtered and Correlative Light-Electron Microscopy: Toward a Quantitative Nanoparticle-Cell Analysis
| العنوان: | High-Contrast Imaging of Nanodiamonds in Cells by Energy Filtered and Correlative Light-Electron Microscopy: Toward a Quantitative Nanoparticle-Cell Analysis |
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| المؤلفون: | Theo Lasser, Lorna Hodgson, Paul Verkade, Marco Raabe, Tanja Weil, Katharina Landfester, Judith Mantell, Ingo Lieberwirth, Shen Han |
| المصدر: | Nano Letters Han, S, Raabe, M, Hodgson, L, Mantell, J, Verkade, P, Lasser, T, Landfester, K, Weil, T & Lieberwirth, I 2019, ' High-Contrast Imaging of Nanodiamonds in Cells by Energy Filtered and Correlative Light-Electron Microscopy : Toward a Quantitative Nanoparticle-Cell Analysis ', Nano Letters, vol. 19, no. 3, pp. 2178-2185 . https://doi.org/10.1021/acs.nanolett.9b00752Test |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Letter, particle-cell interactions, Nanoparticle, Contrast Media, nanodiamond, Cell analysis, 02 engineering and technology, localization, law.invention, law, energy filtered transmission electron microscopy, Microscopy, General Materials Science, Nanodiamond, Instrumentation, 0303 health sciences, Vesicle, High contrast imaging, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Fluorescence, nanoparticle quantification, Cell Tracking, Drug delivery, delivery, 0210 nano-technology, Correlative, Materials science, Bioengineering, Nanotechnology, Electrons, Nanodiamonds, 03 medical and health sciences, protein corona, Energy filtered transmission electron microscopy, Humans, fluorescent nanodiamonds, 030304 developmental biology, Fluorescent Dyes, Organelles, Mechanical Engineering, Correlative light-electron microscopy, General Chemistry, energy-filtered transmission electron microscopy, Nanostructures, Microscopy, Electron, Biophysics, Correlative light electron microscopy, Electron microscope, probes, Energy (signal processing), HeLa Cells |
| الوصف: | Fluorescent nanodiamonds (fNDs) represent an emerging class of nanomaterials offering great opportunities for ultrahigh resolution imaging, sensing and drug delivery applications. Their biocompatibility, exceptional chemical and consistent photostability renders them particularly attractive for correlative light-electron microscopy studies providing unique insights into nanoparticle-cell interactions. Herein, we demonstrate a stringent procedure to image and quantify fNDs with a high contrast down to the single particle level in cells. Individual fNDs were directly visualized by energy-filtered transmission electron microscopy, that is, inside newly forming, early endosomal vesicles during their cellular uptake processes as well as inside cellular organelles such as a mitochondrion. Furthermore, we demonstrate the unequivocal identification, localization, and quantification of individual fNDs in larger fND clusters inside intracellular vesicles. Our studies are of great relevance to obtain quantitative information on nanoparticle trafficking and their various interactions with cells, membranes, and organelles, which will be crucial to design-improved sensors, imaging probes, and nanotherapeutics based on quantitative data. |
| وصف الملف: | application/pdf |
| تدمد: | 1530-6992 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53eea8905b41e183fdd035b660d37146Test https://pubmed.ncbi.nlm.nih.gov/30810045Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....53eea8905b41e183fdd035b660d37146 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15306992 |
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