Social stress-enhanced severity of Citrobacter rodentium induced colitis is CCL2-dependent and attenuated by probiotic Lactobacillus reuteri
| العنوان: | Social stress-enhanced severity of Citrobacter rodentium induced colitis is CCL2-dependent and attenuated by probiotic Lactobacillus reuteri |
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| المؤلفون: | Robert S. Easterling, Michael T. Bailey, Mark Lyte, Timothy D. Eubank, James G. Fox, Amy R. Mackos, Nicola Parry, Jeffrey D. Galley |
| المساهمون: | Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Division of Comparative Medicine, Parry, Nicola, Fox, James G |
| المصدر: | Mucosal immunology PMC |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0301 basic medicine, Limosilactobacillus reuteri, Male, Chemokine, Gene Expression, inflammatory monocyte, Severity of Illness Index, Monocytes, Mice, Intestinal mucosa, Cell Movement, pathogen induced colitis, Citrobacter rodentium, Immunology and Allergy, Intestinal Mucosa, Chemokine CCL2, biology, Enterobacteriaceae Infections, Colitis, 3. Good health, iNOS, medicine.anatomical_structure, psychological phenomena and processes, CCL2, endocrine system, Colon, Immunology, macrophage, Inflammatory bowel diseases, Infectious Colitis, behavioral disciplines and activities, Article, Proinflammatory cytokine, Microbiology, 03 medical and health sciences, medicine, Animals, RNA, Messenger, psychological stress, Monocyte, Macrophages, Probiotics, biology.organism_classification, medicine.disease, Lactobacillus reuteri, Mice, Inbred C57BL, 030104 developmental biology, 13. Climate action, TNF-α, Bacterial Translocation, biology.protein, CCR2, Spleen, Stress, Psychological |
| الوصف: | Psychological stressors are known to affect colonic diseases but the mechanisms by which this occurs, and whether probiotics can prevent stressor effects, are not understood. Because inflammatory monocytes that traffic into the colon can exacerbate colitis, we tested whether CCL2, a chemokine involved in monocyte recruitment, was necessary for stressor-induced exacerbation of infectious colitis. Mice were exposed to a social disruption stressor that entails repeated social defeat. During stressor exposure, mice were orally challenged with Citrobacter rodentium to induce a colonic inflammatory response. Exposure to the stressor during challenge resulted in significantly higher colonic pathogen levels, translocation to the spleen, increases in colonic macrophages, and increases in inflammatory cytokines and chemokines. The stressor-enhanced severity of C. rodentium-induced colitis was not evident in CCL2[superscript −/−] mice, indicating the effects of the stressor are CCL2-dependent. In addition, we tested whether probiotic intervention could attenuate stressor-enhanced infectious colitis by reducing monocyte/macrophage accumulation. Treating mice with probiotic Lactobacillus reuteri reduced CCL2 mRNA levels in the colon and attenuated stressor-enhanced infectious colitis. These data demonstrate that probiotic L. reuteri can prevent the exacerbating effects of stressor exposure on pathogen-induced colitis, and suggest that one mechanism by which this occurs is through downregulation of the chemokine CCL2. National Cancer Institute (U.S.) (Grants AT006552-01A1, P30-CA016058, and T32-DE014320) |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1935-3456 1933-0219 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46ae871b59f819cb7469b57909865a7cTest http://europepmc.org/articles/PMC4794400Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....46ae871b59f819cb7469b57909865a7c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19353456 19330219 |
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