RT‐QuIC Detection of Pathological α‐Synuclein in Skin Punches of Patients with Lewy Body Disease
| العنوان: | RT‐QuIC Detection of Pathological α‐Synuclein in Skin Punches of Patients with Lewy Body Disease |
|---|---|
| المؤلفون: | Piero Parchi, Simone Baiardi, Vincenzo Donadio, Sabina Capellari, Corinne Quadalti, Rocco Liguori, Marcello Rossi, Angela Mammana |
| المساهمون: | Mammana A., Baiardi S., Quadalti C., Rossi M., Donadio V., Capellari S., Liguori R., Parchi P. |
| المصدر: | Movement Disorders |
| بيانات النشر: | John Wiley & Sons, Inc., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Lewy Body Disease, Pathology, medicine.medical_specialty, CSF, Regular Issue Articles, prion, 03 medical and health sciences, 0302 clinical medicine, Medicine, Diagnostic biomarker, Humans, prions, Pathological, Skin, Synucleinopathies, integumentary system, business.industry, Brief Report, synucleinopathies, real‐time quaking‐induced conversion, 030104 developmental biology, Neurology, alpha-Synuclein, Biomarker (medicine), biomarker, α synuclein, Brief Reports, Neurology (clinical), Autopsy, business, Lewy body disease, real-time quaking-induced conversion, synucleinopathie, 030217 neurology & neurosurgery, Biomarkers, Human |
| الوصف: | Background: Evidence suggests that skin represents a suitable matrix for demonstrating α-synuclein oligomers as a diagnostic biomarker for Lewy body disease. Objective: The objective of this study was to evaluate the diagnostic performance of skin α-syn real-time quaking-induced conversion assay in patients with Lewy body disease. Methods: We analyzed skin punches taken in vitam (n=69) or postmortem (n=49) from patients with PD, dementia with Lew bodies (DLB), incidental Lewy body pathology, and neurological controls. Seventy-nine patients underwent both CSF and skin α-synuclein real-time quaking-induced conversion assay. Results: Overall, the skin α-synuclein real-time quaking-induced conversion assay distinguished Lewy body disease patients with 94.1% accuracy (sensitivity, 89.2%; specificity, 96.3%). Assay sensitivity reached 94.1% in the 17 Lewy body disease patients analyzed in the cervical region. In patients with both CSF and skin samples, the 2 real-time quaking-induced conversion assay protocols yielded similar diagnostic accuracy (skin, 97.5%; CSF, 98.7%). Conclusion: Skin punch biopsies might represent a valid and convenient alternative to CSF analysis to demonstrate Lew body-related α-synuclein deposition in patients with Lewy body disease. © 2021 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society. |
| وصف الملف: | STAMPA |
| اللغة: | English |
| تدمد: | 1531-8257 0885-3185 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1b10de634087ad936e37b0590ef2a4cTest http://europepmc.org/articles/PMC8518528Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....d1b10de634087ad936e37b0590ef2a4c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15318257 08853185 |
|---|