دورية أكاديمية
A Polyclonal Selex Aptamer Library Directly Allows Specific Labelling of the Human Gut Bacterium Blautia producta without Isolating Individual Aptamers
| العنوان: | A Polyclonal Selex Aptamer Library Directly Allows Specific Labelling of the Human Gut Bacterium Blautia producta without Isolating Individual Aptamers |
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| المؤلفون: | Hu Xing, Yiting Zhang, Markus Krämer, Ann-Kathrin Kissmann, Marius Henkel, Tanja Weil, Uwe Knippschild, Frank Rosenau |
| المصدر: | Molecules, Vol 27, Iss 17, p 5693 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Organic chemistry |
| مصطلحات موضوعية: | aptamer, B. producta, biosensor, Organic chemistry, QD241-441 |
| الوصف: | Recent studies have demonstrated that changes in the abundance of the intestinal bacterium Blautia producta, a potential probiotic, are closely associated with the development of various diseases such as obesity, diabetes, some neurodegenerative diseases, and certain cancers. However, there is still a lack of an effective method to detect the abundance of B. producta in the gut rapidly. Especially, DNA aptamers are now widely used as biometric components for medical testing due to their unique characteristics, including high chemical stability, low production cost, ease of chemical modification, low immunogenicity, and fast reproducibility. We successfully obtained a high-affinity nucleic acid aptamer library (B.p-R14) after 14 SELEX rounds, which efficiently discriminates B. producta in different analysis techniques including fluorometric suspension assays or fluorescence microscopy from other major gut bacteria in complex mixtures and even in human stool samples. These preliminary findings will be the basis towards aptamer-based biosensing applications for the fast and reliable monitoring of B. producta in the human gut microbiome. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 27175693 1420-3049 |
| العلاقة: | https://www.mdpi.com/1420-3049/27/17/5693Test; https://doaj.org/toc/1420-3049Test |
| DOI: | 10.3390/molecules27175693 |
| الوصول الحر: | https://doaj.org/article/6aa4fa4a43054fe0b17cbe9a9f6b69c6Test |
| رقم الانضمام: | edsdoj.6aa4fa4a43054fe0b17cbe9a9f6b69c6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 27175693 14203049 |
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| DOI: | 10.3390/molecules27175693 |