التفاصيل البيبلوغرافية
| العنوان: |
Bacillus cereus Toxin Repertoire: Diversity of (Iso)cereulide(s). |
| المؤلفون: |
Walser, Veronika, Kranzler, Markus, Dawid, Corinna, Ehling-Schulz, Monika, Stark, Timo D., Hofmann, Thomas F. |
| المصدر: |
Molecules; Feb2022, Vol. 27 Issue 3, p872, 1p |
| مصطلحات موضوعية: |
BACILLUS cereus, TOXINS, FOOD contamination, ACID analysis, POISONS, DIPEPTIDES, T cell receptors, FUSARIUM toxins |
| مستخلص: |
The emetic Bacillus cereus toxin cereulide (1) poses a significant safety risk in the food industry, causing emesis and nausea after consumption of contaminated foods. Analogously to cereulide, the structures of various isocereulides, namely, isocereulides A–G, have been recently reported and could also be identified in B. cereus-contaminated food samples. The HPLC fractionation of B. cereus extracts allows us to isolate additional isocereulides. By applying MSn sequencing, post-hydrolytic dipeptide, amino acid and α-hydroxy acid analyses using UPLC-ESI-TOF-MS to purify the analytes, seven new isocereulides H–N (2–8) could be elucidated in their chemical structures. The structure elucidation was supported by one-dimensional and two-dimensional NMR spectra of the isocereulides H (2), K (5), L and N (6 + 8) and M (7). The toxicity of 2–8 was investigated in a HEp-2 cell assay to determine their respective 50% effective concentration (EC50). Thus, 2–8 exhibited EC50 values ranging from a 0.4- to 1.4-fold value compared to cereulide (1). Missing structure-activity correlations indicate the necessity to determine the toxic potential of all naturally present isocereulides as single compounds to be able to perform a thorough toxicity evaluation of B. cereus-contaminated foods in the future. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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