Rat RI? isoform of type I regulatory subunit of cyclic adenosine monophosphate-dependent protein kinase: cDNA sequence analysis, mRNA tissue specificity, and rat/mouse difference in expression in testis
| العنوان: | Rat RI? isoform of type I regulatory subunit of cyclic adenosine monophosphate-dependent protein kinase: cDNA sequence analysis, mRNA tissue specificity, and rat/mouse difference in expression in testis |
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| المؤلفون: | Robert E. Fellows, Richard A. Maurer, John S. Massa |
| المصدر: | Molecular Reproduction and Development. 26:129-133 |
| بيانات النشر: | Wiley, 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Male, Gene isoform, Molecular Sequence Data, Restriction Mapping, Regulatory Sequences, Nucleic Acid, Biology, Mice, chemistry.chemical_compound, Complementary DNA, Testis, Gene expression, Genetics, Animals, Coding region, Cyclic adenosine monophosphate, Amino Acid Sequence, RNA, Messenger, Protein kinase A, Mice, Inbred BALB C, Messenger RNA, Base Sequence, Brain, Cell Biology, Molecular biology, Rats, Open reading frame, chemistry, Protein Kinases, Developmental Biology |
| الوصف: | A rat complementary DNA (cDNA) for the RI beta isoform of type I cyclic adenosine monophosphate (cAMP)-dependent protein kinase regulatory subunit was cloned and sequenced and was found to contain the entire protein coding and 3'-untranslated regions, with a single (ATTAAA) poly-adenylation site. The largest open reading frame was preceded by a short out-of-phase open reading frame, which is not seen in the corresponding mouse RI beta cDNA due to a single base substitution. The rat RI beta cDNA clone was 2,374 bases long and detected a rat mRNA of approximately 2.8 kilobases. Rat RI beta mRNA was abundant in adult rat brain and testis but was undetectable in other rat tissues. The rat RI beta cDNA also detected RI beta mRNA in mouse brain, but not mouse testis, from 10-week-old BALB/c or 10- and 6-week-old Swiss Webster mice. Thus, despite a 96% nucleotide identity in the coding region of RI beta in rat vs. mouse, there are at least two differences in these closely related species. First, there is a short open reading frame, which precedes the coding region in the rat but not the mouse. Second, unlike the mouse testis, the rat testis contains abundant levels of RI beta mRNA. |
| تدمد: | 1098-2795 1040-452X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82c7f89bfce77132eadca586c0eb1f12Test https://doi.org/10.1002/mrd.1080260206Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....82c7f89bfce77132eadca586c0eb1f12 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10982795 1040452X |
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