Local Prevention of Oxidative Stress in the Intestinal Epithelium of the Rat by Adhesive Liposomes of Superoxide Dismutase and Tempamine
| العنوان: | Local Prevention of Oxidative Stress in the Intestinal Epithelium of the Rat by Adhesive Liposomes of Superoxide Dismutase and Tempamine |
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| المؤلفون: | Abraham Rubinstein, Tareq Jubeh, Sue Haupt, Yechezkel Barenholz, Sivan Antler |
| المصدر: | Molecular Pharmaceutics. 2:2-11 |
| بيانات النشر: | American Chemical Society (ACS), 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Male, Xanthine Oxidase, Antioxidant, medicine.medical_treatment, Pharmaceutical Science, Pharmacology, medicine.disease_cause, Antioxidants, Cyclic N-Oxides, Superoxide dismutase, chemistry.chemical_compound, Intestinal mucosa, Cations, Drug Discovery, medicine, Animals, Humans, Cationic liposome, Ferrous Compounds, Intestinal Mucosa, Xanthine oxidase, Hypoxanthine, Liposome, L-Lactate Dehydrogenase, biology, Hydroxyl Radical, Superoxide Dismutase, Chemistry, Adhesiveness, Rats, Inbred Strains, Intestinal epithelium, Rats, Oxidative Stress, Jejunum, Liposomes, Potassium, biology.protein, Feasibility Studies, Molecular Medicine, HT29 Cells, Oxidative stress |
| الوصف: | The purpose of the present study was to investigate whether the local prevention of luminal superoxide-mediated biological damage in the rat jejunal mucosa could be achieved by liposomal superoxide dismutase (SOD) and the SOD mimic tempamine (TMN). Cationic liposomes loaded with either SOD or TMN were perfused in the rat jejunum prior to the induction of oxidative insult. Reactive hydroxyl radicals were generated in situ in a closed circulating intestinal loop of the rat from the reaction between hypoxanthine and xanthine oxidase in the presence of chelated ferrous sulfate. Mucosal activity of lactate dehydrogenase and levels of potassium ions were used to quantify the tissue damage. Intracellular uptake and locality of SOD were examined in HT-29 cells. The intestinal uptake of SOD and TMN was further measured by using rat colon sacs. Entrapment in cationic liposomes was found to significantly enhance the antioxidant effect of SOD and TMN against the induced oxidative damage in the jejunal mucosa, compared with their free forms. The effect was found to be local and was caused by the increased mucosal adhesion of the liposomes. The cationic liposomes also triggered SOD uptake into the HT-29 cell line. It is concluded that the increased residence time of the cationic liposomes of SOD and TMN in the jejunal mucosa resulted in a local effect against oxidative injury. This local protection may be exploited for drug delivery purposes. |
| تدمد: | 1543-8392 1543-8384 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c60927db1757680f15e6778cfc56a7f0Test https://doi.org/10.1021/mp0499095Test |
| رقم الانضمام: | edsair.doi.dedup.....c60927db1757680f15e6778cfc56a7f0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15438392 15438384 |
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