التفاصيل البيبلوغرافية
| العنوان: |
Characterization of a novel organelle in Toxoplasma gondii with similar composition and function to the plant vacuole. |
| المؤلفون: |
Miranda, Kildare, Pace, Douglas A., Cintron, Roxana, Rodrigues, Juliany C. F., Fang, Jianmin, Smith, Alyssa, Rohloff, Peter, Coelho, Elvis, De Haas, Felix, De Souza, Wanderley, Coppens, Isabelle, Sibley, L. David, Moreno, Silvia N. J. |
| المصدر: |
Molecular Microbiology; Jun2010, Vol. 76 Issue 6, p1358-1375, 18p, 9 Diagrams, 2 Charts, 3 Graphs |
| مصطلحات موضوعية: |
TOXOPLASMA gondii, APICOMPLEXA, ORGANELLES, ADENOSINE triphosphatase, CALCIUM ions, PARASITES |
| مستخلص: |
Toxoplasma gondii belongs to the phylum Apicomplexa and is an important cause of congenital disease and infection in immunocompromised patients. Like most apicomplexans, T. gondii possesses several plant-like features, such as the chloroplast-like organelle, the apicoplast. We describe and characterize a novel organelle in T. gondii tachyzoites, which is visible by light microscopy and possesses a broad similarity to the plant vacuole. Electron tomography shows the interaction of this vacuole with other organelles. The presence of a plant-like vacuolar proton pyrophosphatase (TgVP1), a vacuolar proton ATPase, a cathepsin L-like protease (TgCPL), an aquaporin (TgAQP1), as well as Ca2+/H+ and Na+/H+ exchange activities, supports similarity to the plant vacuole. Biochemical characterization of TgVP1 in enriched fractions shows a functional similarity to the respective plant enzyme. The organelle is a Ca2+ store and appears to have protective effects against salt stress potentially linked to its sodium transport activity. In intracellular parasites, the organelle fragments, with some markers colocalizing with the late endosomal marker, Rab7, suggesting its involvement with the endocytic pathway. Studies on the characterization of this novel organelle will be relevant to the identification of novel targets for chemotherapy against T. gondii and other apicomplexan parasites as well. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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