Expression and analyses of the HIF-1 pathway in the lungs of humans with pulmonary arterial hypertension
| العنوان: | Expression and analyses of the HIF-1 pathway in the lungs of humans with pulmonary arterial hypertension |
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| المؤلفون: | Can Chen, Shian Huang, Yuan He, Xiaorong Shui, Yu Zhang, Wei Lei, Guosen Yan, Guoming Li, Yuanlin Ding |
| المصدر: | Molecular Medicine Reports. 14:4383-4390 |
| بيانات النشر: | Spandidos Publications, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, MAPK/ERK pathway, Cancer Research, medicine.medical_specialty, Pathology, Adolescent, MAP Kinase Signaling System, Hypertension, Pulmonary, Biology, Biochemistry, Pathogenesis, Phosphatidylinositol 3-Kinases, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Genetics, medicine, Humans, Phosphorylation, Protein kinase A, Lung, Molecular Biology, Protein kinase B, PI3K/AKT/mTOR pathway, Mitogen-Activated Protein Kinase 1, Kinase, Aryl Hydrocarbon Receptor Nuclear Translocator, Middle Aged, Hypoxia (medical), Hypoxia-Inducible Factor 1, alpha Subunit, 030104 developmental biology, Endocrinology, Gene Expression Regulation, Oncology, Hypoxia-inducible factors, 030220 oncology & carcinogenesis, Molecular Medicine, Female, medicine.symptom, Proto-Oncogene Proteins c-akt |
| الوصف: | Pulmonary arterial hypertension (PAH) is characterized by endothelial dysfunction and structural remodeling of the pulmonary vasculature, mediated initially by reduced oxygen availability in the lungs. Hypoxia inducible factor (HIF), consisting of the functional subunit, HIF‑1α, and the constitutively expressed HIF‑1β, is involved in the pathological processes associated with hypoxia. In the current study, the sequences of cDNAs and amino acids of HIF were characterized and analyzed using online bioinformatics tools. To further evaluate whether HIF accounts for the occurrence of PAH, the present study determine the expression and phosphorylation levels of HIF and its associated pathways, including extracellular signal‑regulated kinase (Erk)1/2 and phosphoinositide 3‑kinase (PI3K)/Akt, in the lungs of patients with PAH by reverse transcription‑quantitative polymerase chain reaction and western blotting. The mRNA expression levels of PI3K, Erk2, and HIF‑1α in the patients with PAH were significantly higher, compared with those in the control group, by 3.6‑fold (P0.01), 4.06‑fold and 2.64‑fold (P0.05), respectively. No significant differences were found in the mRNA and protein levels of Akt between the two groups (P0.05). The protein levels of phosphorylated (p‑)Akt, Erk1/2, p‑Erk1/2, HIF‑1α and HIF‑1β were significantly increased by 5.89‑, 0.5‑, 0.59‑, 1.46‑ and 0.92‑fold, respectively, in the patients with PAH, compared with those in the controls group (P0.01 for p‑Akt, Erk1/2; P0.05 for p‑Erk1/2, HIF‑1α and HIF‑1β). These findings suggested that the mitogen‑activated protein kinase and PI3K/Akt signaling pathways, and HIF‑1 may perform a specific function in the pathogenesis of PAH. |
| تدمد: | 1791-3004 1791-2997 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a786fd661015c2c9cd4b744ab72b66f2Test https://doi.org/10.3892/mmr.2016.5752Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a786fd661015c2c9cd4b744ab72b66f2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17913004 17912997 |
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