دورية أكاديمية
Expression of Immunoglobulin Receptors with Distinctive Features Indicating Antigen Selection by Marginal Zone B Cells from Human Spleen
| العنوان: | Expression of Immunoglobulin Receptors with Distinctive Features Indicating Antigen Selection by Marginal Zone B Cells from Human Spleen |
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| المؤلفون: | Monica Colombo, Giovanna Cutrona, Daniele Reverberi, Silvia Bruno, Fabio Ghiotto, Claudya Tenca, Kostas Stamatopoulos, Anastasia Hadzidimitriou, Jenny Ceccarelli, Sandra Salvi, Simona Boccardo, Maria Grazia Calevo, Amleto De Santanna, Mauro Truini, Franco Fais, Manlio Ferrarini |
| المصدر: | Molecular Medicine, Vol 19, Iss 1, Pp 294-302 (2013) |
| بيانات النشر: | BMC, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Therapeutics. Pharmacology LCC:Biochemistry |
| مصطلحات موضوعية: | Antigen Selection, Intraclonal Diversification, Recurrent Sequence, Activation-induced Cytidine Deaminase (AID), Follicular Mantle (FM), Therapeutics. Pharmacology, RM1-950, Biochemistry, QD415-436 |
| الوصف: | Abstract Marginal zone (MZ) B cells, identified as surface (s)IgMhighsIgDlowCD23low/−CD21+CD38− B cells, were purified from human spleens, and the features of their V(D)J gene rearrangements were investigated and compared with those of germinal center (GC), follicular mantle (FM) and switched memory (SM) B cells. Most MZ B cells were CD27+ and exhibited somatic hypermutations (SHM), although to a lower extent than SM B cells. Moreover, among MZ B-cell rearrangements, recurrent sequences were observed, some of which displayed intraclonal diversification. The same diversifying sequences were detected in very low numbers in GC and FM B cells and only when a highly sensitive, gene-specific polymerase chain reaction was used. This result indicates that MZ B cells could expand and diversify in situ and also suggested the presence of a number of activation-induced cytidine deaminase (AID)-expressing B cells in the MZ. The notion of antigen-driven expansion/selection in situ is further supported by the VH CDR3 features of MZ B cells with highly conserved amino acids at specific positions and by the finding of shared (“stereotyped”) sequences in two different spleens. Collectively, the data are consistent with the notion that MZ B cells are a special subset selected by in situ antigenic stimuli. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1076-1551 1528-3658 |
| العلاقة: | https://doaj.org/toc/1076-1551Test; https://doaj.org/toc/1528-3658Test |
| DOI: | 10.2119/molmed.2013.00069 |
| الوصول الحر: | https://doaj.org/article/5ff8312866684365b9dfee629c1b0256Test |
| رقم الانضمام: | edsdoj.5ff8312866684365b9dfee629c1b0256 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10761551 15283658 |
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| DOI: | 10.2119/molmed.2013.00069 |