Molecular Cloning of the Human Leukotriene C4 Synthase Gene and Assignment to Chromosome 5q35
| العنوان: | Molecular Cloning of the Human Leukotriene C4 Synthase Gene and Assignment to Chromosome 5q35 |
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| المؤلفون: | Lixia Fu, Craig R. Hodulik, Timothy D. Bigby, Karen C. Arden |
| المصدر: | Molecular Medicine. 2:637-646 |
| بيانات النشر: | Springer Science and Business Media LLC, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Genetics, CAAT box, Molecular cloning, Biology, Molecular biology, Exon, Subcloning, Complementary DNA, Molecular Medicine, Coding region, Genomic library, Molecular Biology, Gene, Genetics (clinical) |
| الوصف: | Cysteinyl leukotrienes (LT) are mediators involved in inflammatory and allergic disorders. LTC4 synthase catalyzes the first committed step in the synthesis of these inflammatory mediators, and its cellular distribution appears to be unique. A human genomic library was screened by polymerase chain reaction (PCR) with primers that were designed based on the reported cDNA sequence for the LTC4 synthase gene. The gene was identified in one clone by Southern blotting of restriction enzyme digests, subcloning of fragments containing regions of interest, and DNA sequencing of these sub-clones. The transcription initiation site was detennined by primer extension analysis. Chromosome location was determined by fluorescent in situ hybridization and screening of somatic cell hybrids by PCR. The LTC4 synthase gene is ∼2.5 kb in length, consisting of five exons (136, 100, 71, 82, and 257 bp, respectively) and four introns (1,447, 102, 84, and 230 bp, respectively). Transcription initiation occurs at a single site 78 bp upstream of the coding region. The 5′-flanking region contains neither a TATA nor a CAAT box. The first 1 kb of the 5′-flanking region, however, contains putative DNA binding motifs for SP-1, AP-1, AP-2, ets factors, and CREB/ATF. A STAT binding motif is present in the first intron. The LTC4 synthase gene is located in the distal region of the long arm of chromosome 5 in 5q35. The LTC4 synthase gene does not contain elements of a typical regulated gene and may therefore contain novel regulatory elements. This gene is also located in a region on chromosome 5 that appears to play a role in allergic and inflammatory disorders, such as asthma. |
| تدمد: | 1528-3658 1076-1551 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::3aa35412e2b9c57eee765bb7a1ecf889Test https://doi.org/10.1007/bf03401647Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........3aa35412e2b9c57eee765bb7a1ecf889 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15283658 10761551 |
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