المؤلفون: Manoel L, DA Fontoura-Rodrigues, Carlos André V, Lima-Rosa, Ligia, Tchaicka, Fernanda P, Valdez, Flávio H G, Rodrigues, Rogério C, DE Paula, Michael P, Gough, Warren E, Johnson, Sandro L, Bonatto, Eduardo, Eizirik

المصدر: Molecular ecology resources. 8(4)

الوصف: We tested 47 tetranucleotide microsatellite loci developed for the domestic dog in four species of Neotropical canids, aiming to produce a standardized set that could be successfully used even in noninvasive samples across this group. We identified 13 suitable loci, nine of which constitute a standardized set for all species. Considering only the ideal panel of nine loci, the mean expected heterozygosity (averaged across species) per locus ranged from 0.58 to 0.92 (overall mean 0.76), and the maximum probability of identity value was 1.3 × 10(-9) . This set of loci has a great potential for application in evolutionary, ecological and conservation studies.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::cbf798962364eb6d482ca45ec4d23330Test
https://pubmed.ncbi.nlm.nih.gov/21585923Test

المؤلفون: DA FONTOURA-RODRIGUES, MANOEL L.^1,2, LIMA-ROSA, CARLOS ANDRÉ V.^1,2, TCHAICKA, LIGIA^1,2, VALDEZ, FERNANDA P.^1,2, RODRIGUES, FLÁVIO H. G.^3,4, DE PAULA, ROGÉRIO C.^3,5, GOUGH, MICHAEL P.⁶, JOHNSON, WARREN E.⁶, BONATTO, SANDRO L.¹, EIZIRIK, EDUARDO^1,3,6 eduardo.eizirik@pucrs.br

المصدر: Molecular Ecology Resources. Jul2008, Vol. 8 Issue 4, p898-900. 3p. 2 Charts.

مصطلحات موضوعية: *DOGS, *ANIMAL species, *MICROSATELLITE repeats, *PROBABILITY theory, *ECOLOGY, *CONSERVATION of natural resources

مستخلص: We tested 47 tetranucleotide microsatellite loci developed for the domestic dog in four species of Neotropical canids, aiming to produce a standardized set that could be successfully used even in noninvasive samples across this group. We identified 13 suitable loci, nine of which constitute a standardized set for all species. Considering only the ideal panel of nine loci, the mean expected heterozygosity (averaged across species) per locus ranged from 0.58 to 0.92 (overall mean 0.76), and the maximum probability of identity value was 1.3 × 10−9. This set of loci has a great potential for application in evolutionary, ecological and conservation studies. [ABSTRACT FROM AUTHOR]