Hypercholesterolemia in rats impairs the cholinergic system and leads to memory deficits
| العنوان: | Hypercholesterolemia in rats impairs the cholinergic system and leads to memory deficits |
|---|---|
| المؤلفون: | Michael Pirchl, Celine Ullrich, Christian Humpel |
| المصدر: | Molecular and Cellular Neurosciences |
| بيانات النشر: | Academic Press, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | medicine.medical_specialty, Blotting, Western, Hypercholesterolemia, Inflammation, Beta-amyloid(1–42), Enzyme-Linked Immunosorbent Assay, tau Proteins, Cholinergic dysfunction, Biology, Article, Rats, Sprague-Dawley, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Alzheimer Disease, Internal medicine, Cortex (anatomy), In vivo, medicine, Memory impairment, Animals, Hyperphosphorylated tau, Cholinergic neuron, Maze Learning, Molecular Biology, 030304 developmental biology, Neurons, 0303 health sciences, Memory Disorders, Microbleeding, Amyloid beta-Peptides, Brain, Cell Biology, Alzheimer's disease, medicine.disease, Immunohistochemistry, Rats, Endocrinology, Nerve growth factor, medicine.anatomical_structure, Cholesterol, Cholinergic, medicine.symptom, 030217 neurology & neurosurgery, Acetylcholine, medicine.drug |
| الوصف: | Alzheimer's disease (AD) is a chronic brain disorder characterized by cognitive impairment, cholinergic dysfunction, inflammation, tau and beta-amyloid pathology and vascular damage. Recent studies have shown, that high cholesterol levels are linked to the pathology of AD. The aim of our present work was to study the effects of hypercholesterolemia in adult rats. Five months after 5% cholesterol-enriched diet plasma cholesterol levels and total weight were significantly enhanced compared to controls. Spatial memory was studied in an 8-arm radial maze and cholesterol-treated rats showed an impaired learning and long-term memory. Hypercholesterolemia significantly reduced the number of cholinergic neurons in the basal nucleus of Meynert and decreased acetylcholine levels in the cortex. Nerve growth factor was only slightly enhanced in the cortex of cholesterol-treated animals. Levels of amyloid precursor protein, beta-amyloid(1–42), as well as tau and phospho-tau 181 were significantly enhanced in the cortex of cholesterol-fed rats. Hypercholesterolemia markedly increased several cerebral inflammatory markers and enhanced microglial CD11b-like immunoreactivity. Vascular density, stained by RECA-1 was not changed. However, cholesterol induced cortical microbleedings illustrated by intensive anti-rat IgG-positive spots in the cortex. In conclusion, our data demonstrate that hypercholesterolemia in rats caused memory impairment, cholinergic dysfunction, inflammation, enhanced cortical beta-amyloid and tau and microbleedings, all indications, which resemble an AD-like pathology. |
| اللغة: | English |
| تدمد: | 1095-9327 1044-7431 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abc2554780ae9b6a00aa862219320c4fTest http://europepmc.org/articles/PMC2977849Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....abc2554780ae9b6a00aa862219320c4f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10959327 10447431 |
|---|