Multiple calmodulin mRNA species are derived from two distinct genes
| العنوان: | Multiple calmodulin mRNA species are derived from two distinct genes |
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| المؤلفون: | Koichiro Kishi, Hirofumi Sokabe, Hiroshi Nojima |
| المصدر: | Molecular and Cellular Biology. 7:1873-1880 |
| بيانات النشر: | Informa UK Limited, 1987. |
| سنة النشر: | 1987 |
| مصطلحات موضوعية: | Sequence analysis, Pseudogene, Biology, Calmodulin, Sequence Homology, Nucleic Acid, Complementary DNA, Animals, Coding region, Tissue Distribution, Genomic library, RNA, Messenger, Molecular Biology, Gene, Genomic organization, Pharmacology, Genetics, Base Sequence, cDNA library, Muscles, Single-Strand Specific DNA and RNA Endonucleases, Brain, Chromosome Mapping, DNA, DNA Restriction Enzymes, Cell Biology, Endonucleases, Molecular biology, Rats, Gene Expression Regulation, Genes, Liver, Research Article |
| الوصف: | We have observed three calmodulin mRNA species in rat tissues. In order to know from how many expressed genes they are derived, we have investigated the genomic organization of calmodulin genes in the rat genome. From a rat brain cDNA library, we obtained two kinds of cDNAs (pRCM1 and pRCM3) encoding authentic calmodulin. DNA sequence analysis of these cDNA clones revealed substitutions of nucleotides at 73 positions of 450 nucleotides in the coding region, although the amino acid sequences of these calmodulins are exactly the same. DNA sequences in the 5' and 3' noncoding regions are quite different between these two cDNAs. From these results, we conclude that they are derived from two distinct bona fide calmodulin genes, CaMI (pRCM1) and CaMII (pRCM3). Total genomic Southern hybridization suggested four distinct calmodulin-related genes in the rat genome. By cloning and sequencing the calmodulin-related genes from rat genomic libraries, we demonstrated that the other two genes are processed pseudogenes generated from the CaMI (lambda SC9) and CaMII (lambda SC8) genes, respectively, through an mRNA-mediated process of insertions. Northern blotting showed that the CaMI gene is transcribed in liver, muscle, and brain in similar amounts, whereas the CaMII gene is transcribed mainly in brain. S1 nuclease mapping indicated that the CaMI gene produced two mRNA species (1.7 and 4 kilobases), whereas the CaMII gene expressed a single mRNA species (1.4 kilobases). |
| تدمد: | 1098-5549 0270-7306 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87c723f5f43cc7cf25bcc0e7f440e879Test https://doi.org/10.1128/mcb.7.5.1873Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....87c723f5f43cc7cf25bcc0e7f440e879 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985549 02707306 |
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