Insulin growth factor-1 pathway in cervical carcinoma cancer stem cells
| العنوان: | Insulin growth factor-1 pathway in cervical carcinoma cancer stem cells |
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| المؤلفون: | Rashmi Bagga, Radhika Srinivasan, Shalmoli Bhattacharyya, Shifa Javed |
| المصدر: | Molecular and Cellular Biochemistry. 473:51-62 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Epithelial-Mesenchymal Transition, medicine.medical_treatment, Clinical Biochemistry, Cell, Uterine Cervical Neoplasms, Biology, 03 medical and health sciences, 0302 clinical medicine, Cancer stem cell, Cell Line, Tumor, Gene expression, medicine, Humans, Insulin-Like Growth Factor I, Molecular Biology, Cisplatin, Cervical cancer, Growth factor, Cell Biology, General Medicine, medicine.disease, Neoplasm Proteins, 030104 developmental biology, medicine.anatomical_structure, Apoptosis, Cell culture, 030220 oncology & carcinogenesis, Neoplastic Stem Cells, Cancer research, Female, Signal Transduction, medicine.drug |
| الوصف: | Cancer stem cells (CSC) drive tumour progression and are implicated in relapse and resistance to conventional cancer therapies. Identification of differentially expressed genes by gene expression (GEP) profiling may help identify the differentially activated signalling pathways in cancer stem cells as opposed to bulk tumour cells which will provide new insights into cancer stem cell biology and aid in identification of novel therapeutic targets. Our study focused on the inhibition of CSC from cervical cancer cell lines by targeting insulin-like growth factor (IGF), which was identified by differential GEP. Targeted inhibition of IGF-1 by JB-1 trifluoroacetate (inhibitor of IGF) was carried out in SiHa, RSBS-14 and RSBS-43 cervical cancer derived cell lines. Effect of cisplatin was also evaluated. Inhibition of IGF-1 signalling was confirmed by demonstration of reduction in p-Akt levels. The cell biological effects of IGF-1 inhibition included an increase in G2M/S fraction, increased apoptosis and decreased invasive ability. JB-1 and cisplatin showed synergism. However, transcript levels of stemness and EMT markers showed variable levels following IGF inhibition. Overall, this proof-of-concept study has shown that IGF-1 is an attractive target for inhibition of CSC in invasive cervical cancer. |
| تدمد: | 1573-4919 0300-8177 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::619fe4a597bb20186cf48bf314ac728cTest https://doi.org/10.1007/s11010-020-03807-6Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....619fe4a597bb20186cf48bf314ac728c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15734919 03008177 |
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