FATP1 localizes to mitochondria and enhances pyruvate dehydrogenase activity in skeletal myotubes

التفاصيل البيبلوغرافية
العنوان: FATP1 localizes to mitochondria and enhances pyruvate dehydrogenase activity in skeletal myotubes
المؤلفون: Ester Quintana, Cèlia García-Martínez, Antonio L. Andreu, Elena García-Arumí, Anna M. Gómez-Foix, Paz Briones, Maria Guitart
المصدر: Mitochondrion. 9(4)
سنة النشر: 2008
مصطلحات موضوعية: Pyruvate decarboxylation, Pyruvate dehydrogenase kinase, Protein subunit, Green Fluorescent Proteins, Immunoblotting, Muscle Fibers, Skeletal, Ketone Oxidoreductases, Cell Biology, Biology, Mitochondrion, Pyruvate dehydrogenase phosphatase, Pyruvate dehydrogenase complex, Cell Fractionation, Fatty Acid Transport Proteins, Transport protein, Mitochondria, Biochemistry, Microscopy, Fluorescence, Genes, Reporter, Molecular Medicine, Humans, Muscle, Skeletal, Molecular Biology, Pyruvate decarboxylase, Cells, Cultured
الوصف: Fatty acid transport protein 1 (FATP1) has been previously immunolocalized in intracellular compartments. Here we show that FATP1 localizes to the mitochondria in cultured myotubes, by immunoblots of subcellular fractions and immunocytology of the fusion protein FATP1-GFP. FATP1 strongly stimulates CO(2) production from glucose whereas nonmitochondrial metabolism of glucose is only slightly enhanced. FATP1 raises the activity and activates the pyruvate dehydrogenase (PDH) complex and the pyruvate decarboxylase PDH-E1 catalytic subunit, without changing E2, E3BP or E1alpha and increasing E1beta protein content. These data reveals the localization and points to a regulatory function of FATP1 in myotube mitochondria.
تدمد: 1872-8278
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2106e088e35e52bc248c6eb1f8a7b667Test
https://pubmed.ncbi.nlm.nih.gov/19361580Test
حقوق: CLOSED
رقم الانضمام: edsair.doi.dedup.....2106e088e35e52bc248c6eb1f8a7b667
قاعدة البيانات: OpenAIRE