التفاصيل البيبلوغرافية
| العنوان: |
Intranucleolar localization of DNA topoisomerase IIα is a distinctive feature of necrotic, but not of apoptotic, Jurkat T-cells. |
| المؤلفون: |
Elisabetta Falcieri, Sabrina Burattini, Roberta Bortul, Francesca Luchetti, Giovanna Tabellini, Pier Luigi Tazzari, Alessandra Cappellini, Lucio Cocco |
| المصدر: |
Microscopy Research & Technique; Oct2003, Vol. 62 Issue 3, p192, 9p |
| مصطلحات موضوعية: |
APOPTOSIS, NECROSIS, DNA topoisomerases |
| مستخلص: |
Two distinct types of cell death have been described: apoptosis and necrosis. However, it is becoming increasingly clear that the differences between these two types are far less numerous than initially thought. Morphological analyses might provide important information to distinguish apoptotic from necrotic samples. We recently reported that in necrotic, but not apoptotic, HL-60 human myeloid leukaemia cells, the nuclear protein topoisomerase IIα concentrated in nucleoli. In order to ascertain whether or not this phenomenon was restricted to a peculiar cell type or could be detected also in cells of lymphoid lineage, we performed an investigation aimed at defining the localization of topoisomerase IIα in apoptotic and necrotic Jurkat human T lymphoblastoid cells. Immunofluorescence staining demonstrated that topoisomerase IIα was excluded from the condensed chromatin of apoptotic cells, whereas in necrotic cells it was localized in discrete nuclear dots. Immuno-electron microscopy analysis showed that topoisomerase IIα was undetectable in nucleoli of normal and apoptotic cells, whereas it was present in the nucleolus of necrotic cells irrespectively of the type of inducer used (ethanol, H2O2, HgCl2). Taken together, our findings identify topoisomerase IIα as a potential morphological marker useful to discriminate between apoptotic and necrotic cells. Microsc. Res. Tech. 62:192200, 2003. © 2003 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
