التفاصيل البيبلوغرافية
| العنوان: |
Characterization of the Duodenal Mucosal Microbiome in Obese Adult Subjects by 16S rRNA Sequencing. |
| المؤلفون: |
Nardelli, Carmela, Granata, Ilaria, D'Argenio, Valeria, Tramontano, Salvatore, Compare, Debora, Guarracino, Mario Rosario, Nardone, Gerardo, Pilone, Vincenzo, Sacchetti, Lucia |
| المصدر: |
Microorganisms; Apr2020, Vol. 8 Issue 4, p485, 1p |
| مصطلحات موضوعية: |
RECEIVER operating characteristic curves, OVERWEIGHT persons, GUT microbiome |
| مصطلحات جغرافية: |
CAMPANIA (Italy), ITALY |
| مستخلص: |
The gut microbiota may have an impact on obesity. To date, the majority of studies in obese patients reported microbiota composition in stool samples. The aim of this study was to investigate the duodenal mucosa dysbiosis in adult obese individuals from Campania, a region in Italy with a very high percentage of obese people, to highlight microbial taxa likely associated with obesity. Duodenum biopsies were taken during upper gastrointestinal endoscopy in 19 obese (OB) and 16 lean control subjects (CO) and microbiome studied by 16S rRNA gene sequencing. Duodenal microbiome in our groups consisted of six phyla: Proteobacteria, Firmicutes, Actinobacteria, Fusobacteria, Bacteroidetes and Acidobacteria. Proteobacteria (51.1% vs. 40.1%) and Firmicutes (33.6% vs. 44.9%) were significantly (p < 0.05) more and less abundant in OB compared with CO, respectively. Oribacterium asaccharolyticum, Atopobium parvulum and Fusobacterium nucleatum were reduced (p < 0.01) and Pseudomonadales were increased (p < 0.05) in OB compared with CO. Receiver operating characteristic curve analysis showed Atopobium and Oribacterium genera able to discriminate with accuracy (power = 75% and 78%, respectively) OB from CO. In conclusion, increased Proteobacteria and decreased Firmicutes (Lachnospiraceae) characterized the duodenal microbiome of obese subjects. These data direct to further studies to evaluate the functional role of the dysbiotic-obese-associated signature. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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