Emergence of a Novel Ehrlichia minasensis Strain, Harboring the Major Immunogenic Glycoprotein trp36 with Unique Tandem Repeat and C-Terminal Region Sequences, in Haemaphysalis hystricis Ticks Removed from Free-Ranging Sheep in Hainan Province, China
| العنوان: | Emergence of a Novel Ehrlichia minasensis Strain, Harboring the Major Immunogenic Glycoprotein trp36 with Unique Tandem Repeat and C-Terminal Region Sequences, in Haemaphysalis hystricis Ticks Removed from Free-Ranging Sheep in Hainan Province, China |
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| المؤلفون: | Xibing Yu, Renfu Yin, Yidong Fei, Jiaqi Mu, Junjiao Li, Yuhai Bi, Yulong Zhou, Jin Chang, Xinxin Liu, Zhuang Ding |
| المصدر: | Microorganisms, Vol 7, Iss 9, p 369 (2019) Microorganisms Volume 7 Issue 9 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Ehrlichia minasensis, Ehrlichia canis, Sequence analysis, 030231 tropical medicine, Tick, Microbiology, Haemaphysalis hystricis tick, 03 medical and health sciences, 0302 clinical medicine, Tandem repeat, Virology, parasitic diseases, South China, lcsh:QH301-705.5, Genetics, biology, Phylogenetic tree, Strain (biology), trp36, biology.organism_classification, 030104 developmental biology, lcsh:Biology (General), GenBank, free-ranging sheep, Haemaphysalis hystricis |
| الوصف: | Ehrlichia minasensis, a recently described Ehrlichia species that is the most closely related to, but clearly distinct from, Ehrlichia canis, has been circulating in not only bovines, cervids, and dogs but also several tick species from Canada, Brazil, France, Pakistan, Ethiopia, and Israel. However, there are no reports of E. minasensis in China. The purpose of this study was to explore whether E. minasensis is present naturally in ticks in China. Through PCR targeting of the genus-conserved dsb gene, E. minasensis DNA was detected in Haemaphysalis hystricis ticks removed from free-ranging sheep in Hainan Province, South China in 2017. The partial sequence of the dsb, 16S rRNA, and groEL genes demonstrated that the Hainan strain shared 99% identity with the dsb gene of E. minasensis strain UFMG-EV (GenBank: JX629808), with the 16S rRNA of E. minasensis isolate E-2650 (MH500005) and with the groEL gene of E. minasensis strain UFMG-EV (JX629806), respectively. Moreover, sequence analysis of the major immunogenic tandem repeat protein (trp36) revealed that the Hainan strain harbored a unique tandem repeat sequence (APEAAPVSAPEAAPVSAPVS) and a C-terminal region that differed from those of other known E. minasensis strains. Additionally, phylogenetic analysis based on the entire amino acid sequence of trp36 revealed that the Hainan strain was closely related to a recently described E. minasensis strain from Brazil, of which the sister clade contained different strains of E. canis. The discovery of this novel Hainan strain in H. hystricis ticks represents the first known natural presence of E. minasensis in South China, highlighting the need for its constant surveillance. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-2607 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fadf59e49830c63a56d6959222ccc459Test https://www.mdpi.com/2076-2607/7/9/369Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fadf59e49830c63a56d6959222ccc459 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20762607 |
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