Adaptive and Innate Immune Cells in Fetal Human Cytomegalovirus-Infected Brains
| العنوان: | Adaptive and Innate Immune Cells in Fetal Human Cytomegalovirus-Infected Brains |
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| المؤلفون: | Férechté Encha-Razavi, Tiffany Guilleminot, Julien Stirnemann, Bettina Bessières, Yann Sellier, Nacilla Haicheur, Franck Pagès, Yves Ville, Florence Marliot, Marianne Leruez-Ville |
| المصدر: | Microorganisms, Vol 8, Iss 2, p 176 (2020) Microorganisms Volume 8 Issue 2 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), Human cytomegalovirus, lag-3, 030106 microbiology, Brain damage, Biology, Microbiology, Article, 03 medical and health sciences, Immune system, immune cells, fetal brain, Virology, Immunochemistry, exhaustion, medicine, cytomegalovirus, lcsh:QH301-705.5, Innate immune system, pd-1, medicine.disease, Immune checkpoint, 030104 developmental biology, lcsh:Biology (General), Immunology, medicine.symptom, Ex vivo, CD8 |
| الوصف: | Background: The understanding of the pathogenesis of cytomegalovirus (CMV)-induced fetal brain lesions is limited. We aimed to quantify adaptive and innate immune cells and CMV-infected cells in fetal brains with various degrees of brain damage. Methods: In total, 26 archived embedded fetal brains were studied, of which 21 were CMV-infected and classified in severely affected (n = 13) and moderately affected (n = 8), and 5 were uninfected controls. The respective magnitude of infected cells, immune cells (CD8+, B cells, plasma cells, NK cells, and macrophages), and expression of immune checkpoint receptors (PD-1/PD-L1 and LAG-3) were measured by immunochemistry and quantified by quantitative imaging analysis. Results: Quantities of CD8+, plasma cells, NK cells, macrophages, and HCMV+ cells and expression of PD-1/PD-L1 and LAG-3 were significantly higher in severely affected than in moderately affected brains (all p values < 0.05). A strong link between higher number of stained cells for HCMV/CD8 and PD-1 and severity of brain lesions was found by component analysis. Conclusions: The higher expression of CD8, PD-1, and LAG-3 in severely affected brains could reflect immune exhaustion of cerebral T cells. These exhausted T cells could be ineffective in controlling viral multiplication itself, leading to more severe brain lesions. The study of the functionality of brain leucocytes ex vivo is needed to confirm this hypothesis. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2076-2607 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52d843b690edad855c548edcbffdc25fTest https://www.mdpi.com/2076-2607/8/2/176Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....52d843b690edad855c548edcbffdc25f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20762607 |
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