دورية
Characterization of Monoclonal Antibodies against Etiological Agents of Weil's Disease
| العنوان: | Characterization of Monoclonal Antibodies against Etiological Agents of Weil's Disease |
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| المؤلفون: | Kobayashi, Yuzuru, Tamai, Tomonori, Oyama, Takashi, Hasegawa, Hitoshi, Sada, Eiji, Kusaba, Tomohiro, Hamaji, Masaharu |
| المصدر: | Microbiology and Immunology; March 1984, Vol. 28 Issue: 3 p359-370, 12p |
| مستخلص: | Monoclonal antibodies against etiological agents of Weil's disease were produced by cell fusion technology. Twenty hybridomas were produced through the fusion of P3×63Ag8.653 cells with spleen cells from BALB/c mice immunized against Leptospira interrogansserovar icterohaemorrhagiaeRGA strain and serovar copenhageniShiromizu and M20 strains. Reactivities of the antibodies produced by the hybridomas were determined by the microscopic agglutination test. Among the five hybridoma antibodies to the RGA strain, two reacted specifically to serovar icterohaemorrhagiae, two reacted to serovar icterohaemorrhagiaeat a high titer and serovar copenhageniat a low titer, and one reacted to serovars icterohaemorrhagiae, copenhageni, pyrogenes, and canicola. Of the ten hybridoma antibodies to the Shiromizu strain, one reacted specifically to serovar copenhageni, seven reacted to both serovars copenhageniand icterohaemorrhagiaeat almost the same titer, and two exhibited intermediate properties. Of the five hybridoma antibodies to the M20 strain, three reacted to both serovars copenhageniand icterohaemorrhagiaeat almost the same titer, one reacted to serovar copenhageniat a low titer and serovar icterohaemorrhagiaeat a high titer, and one reacted to serovars copenhageni, icterohaemorrhagiae, and pyrogenes. The results revealed that each serovar has its own antigen(s) and their common antigens. In addition, 20 strains of leptospires were recently isolated and tested with three monoclonal antibodies characterized by different reactivities. Twenty strains were clearly identified by their antibodies, i.e., 16 strains were identified as serovar icterohaemorrhagiaeand three strains were identified as serovar copenhageni. The remaining strain, which was not agglutinated by three antibodies, was identified as serovar autumnalisby an agglutination test with immune rabbit sera. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 03855600 13480421 |
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| DOI: | 10.1111/j.1348-0421.1984.tb00687.x |