The rhizosphere-competent entomopathogen Metarhizium anisopliae expresses a specific subset of genes in plant root exudate
| العنوان: | The rhizosphere-competent entomopathogen Metarhizium anisopliae expresses a specific subset of genes in plant root exudate |
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| المؤلفون: | Sibao Wang, Claudia Angelini, Weiguo Fang, Francisco Posada, Raymond J. St. Leger, Monica Pava-Ripoll |
| المصدر: | Microbiology. 157:47-55 |
| بيانات النشر: | Microbiology Society, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Trichoderma, Exudate, Metarhizium, Rhizosphere, biology, Plant Extracts, Gene Expression Profiling, fungi, Trichoderma harzianum, Beauveria bassiana, Metarhizium anisopliae, Fungi imperfecti, Bassiana, Microarray Analysis, biology.organism_classification, Plant Roots, Microbiology, Gene Expression Regulation, Fungal, medicine, Metarhizium acridum, Aspergillus niger, Beauveria, medicine.symptom |
| الوصف: | Metarhizium anisopliae and Beauveria bassiana are ubiquitous insect pathogens and possible plant symbionts, as some strains are endophytic or colonize the rhizosphere. We evaluated 11 strains of M. anisopliae and B. bassiana, and two soil saprophytes (the non-rhizospheric Aspergillus niger and the rhizosphere-competent Trichoderma harzianum) for their ability to germinate in bean root exudates (REs). Our results showed that some generalist strains of M. anisopliae were as good at germinating in RE as T. harzianum, although germination rates of the specialized acridid pathogen Metarhizium acridum and the B. bassiana strains were significantly lower. At RE concentrations of −1, M. anisopliae strain ARSEF 2575 showed higher germination rates than T. harzianum. Microarrays showed that strain 2575 upregulated 29 genes over a 12 h period in RE. A similar number of genes (21) were downregulated. Upregulated genes were involved in carbohydrate metabolism, lipid metabolism, cofactors and vitamins, energy metabolism, proteolysis, extracellular matrix/cell wall proteins, transport proteins, DNA synthesis, the sexual cycle and stress response. However, 41.3 % of the upregulated genes were hypothetical or orphan sequences, indicating that many previously uncharacterized genes have functions related to saprophytic survival. Genes upregulated in response to RE included the subtilisin Pr1A, which is also involved in pathogenicity to insects. However, the upregulated Mad2 adhesin specifically mediates adhesion to plant surfaces, demonstrating that M. anisopliae has genes for rhizosphere competence that are induced by RE. |
| تدمد: | 1465-2080 1350-0872 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7a1c20aa3bec73882a0406f0a9c04e5Test https://doi.org/10.1099/mic.0.042200-0Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b7a1c20aa3bec73882a0406f0a9c04e5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14652080 13500872 |
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