Metabolic properties of Pachysolen tannophilus mutants producing xylitol and ethanol from D-xylose
| العنوان: | Metabolic properties of Pachysolen tannophilus mutants producing xylitol and ethanol from D-xylose |
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| المؤلفون: | N. P. Mikhailova, A. I. Ginak, O. V. Meshcheryakova, O. I. Bolotnikova |
| المصدر: | Microbiology. 84:485-490 |
| بيانات النشر: | Pleiades Publishing Ltd, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | chemistry.chemical_classification, food and beverages, Dehydrogenase, Xylose, Reductase, Xylitol, Applied Microbiology and Biotechnology, Microbiology, Malate dehydrogenase, carbohydrates (lipids), chemistry.chemical_compound, Enzyme, chemistry, Biochemistry, Lactate dehydrogenase, NAD+ kinase |
| الوصف: | Activity of the major enzymes of D-xylose metabolism in the mutants of xylose-utilizing yeasts Pachysolen tannophilus selectively producing xylitol or ethanol was studied. The xylitol-producing strain exhibited low activities of xylitol dehydrogenase, xylose reductase with preferential affinity to NADPH, NAD+-dependent malate dehydrogenase, and cytochrome c oxidase (4.40, 4.80, 1.87, and 0.28 μmol mg−1 min−1, respectively). The cells of the ethanol-producing mutants exhibited elevated activity of NADH/NADPH-xylose reductase, xylitol dehydrogenase, 1-glycerophosphate dehydrogenase, and lactate dehydrogenase to 6.80, 8.60, 4.68, and 16.48 μmol mg−1 min−1, respectively. Effect of the NADPH/NADH imbalance on ethanol production accumulation and xylitol accumulation is discussed. |
| تدمد: | 1608-3237 0026-2617 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::43f6ab02b752b29db0e023ef3a24907bTest https://doi.org/10.1134/s0026261715040050Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........43f6ab02b752b29db0e023ef3a24907b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16083237 00262617 |
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