التفاصيل البيبلوغرافية
| العنوان: |
Three polypeptides screened from phage display random peptide library may be the receptor polypeptide of Mycoplasma genitalium adhesion protein. |
| المؤلفون: |
Deng, Xiangying1, Zhu, Youcong1, Dai, Pei1, Yu, Minjun1, Chen, Liesong1, Zhu, Cuiming1, You, Xiaoxing1, Li, Lingling1, Zeng, Yanhua1 zengyihua21cn@126.com |
| المصدر: |
Microbial Pathogenesis. Jul2018, Vol. 120, p140-146. 7p. |
| مصطلحات موضوعية: |
*POLYPEPTIDES, *MYCOPLASMA, *PATHOGENIC microorganisms, *ENZYME-linked immunosorbent assay, *IMMUNOFLUORESCENCE |
| مستخلص: |
Abstract Mycoplasma genitalium adhesion protein (MgPa) is a major adhesin of M. genitalium , a human pathogen associated with a series of genitourinary tract diseases. MgPa plays a very important role in M. genitalium adhering to the host cells. However, the exact receptor peptides or proteins of MgPa are still poorly understood so far. Three polypeptides (V-H-W-D-F-R-Q-W-W-Q-P-S), (D-W-S-S-W-V -Y-R-D-P-Q-T) and (H-Y-I-D-F-R-W) were previously screened from a phage display random peptide library using recombinant MgPa (rMgPa) as a target molecule. In this study, three polypeptides were artificially synthesized and investigated as to whether they are potential receptors of MgPa. We found that rMgPa specifically bound to three synthesized polypeptides as determined via an indirect enzyme-linked immunosorbent assay (ELISA). Moreover, three polypeptides were further identified by indirect immunofluorescence microscopy (IFM). We confirmed that rMgPa and M. genitalium can adhere to SV-HUC-1 cells in vitro and that anti-rMgPa antibody and three synthesized polypeptides can partially inhibit the adherence of rMgPa and M. genitalium to SV-HUC-1 cells. In summary, these three polypeptides may be the essential receptor peptides of MgPa, and may aid in enhancing the understanding of biological function of MgPa and the possible pathogenic mechanism of M. genitalium. Highlights • Three polypeptides could bind specifically to rMgPa. • Three polypeptides may be the receptors polypeptides of MgPa. • Three polypeptides can block the adhering of rMgPa and M.genitalium to SV-HUC-1 cells. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
