Probiotic Dosing of Ruminococcus flavefaciens Affects Rumen Microbiome Structure and Function in Reindeer
| العنوان: | Probiotic Dosing of Ruminococcus flavefaciens Affects Rumen Microbiome Structure and Function in Reindeer |
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| المؤلفون: | Svein D. Mathiesen, Vincent G. H. Eijsink, Monica A. Sundset, Roderick I. Mackie, Phillip B. Pope, Kirsti E. Præsteng, Lars P. Folkow, Isaac Cann |
| المصدر: | Microbial Ecology. 66:840-849 |
| بيانات النشر: | Springer Science and Business Media LLC, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, Biogas Reactor, Rumen, Molecular Sequence Data, Soil Science, Biology, Fibre Digestion, Microbiology, 03 medical and health sciences, Microbial ecology, Ruminococcus, VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470, Prevotella, Animals, Microbiome, Food science, Cellulose, Rumen Content, Phylogeny, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, 0303 health sciences, Bacteria, Ecology, Bacteroidetes, 030306 microbiology, Microbiota, Probiotics, Rumen Sampling, Biodiversity, biology.organism_classification, VDP::Mathematics and natural science: 400::Basic biosciences: 470, UniFrac, Digestion, Reindeer, Ruminococcaceae |
| الوصف: | This is a post-peer-review, pre-copyedit version of an article published in Microbial Ecology. The final authenticated version is available online at: https://doi.org/10.1007/s00248-013-0279-zTest. Highly cellulolytic bacterial species such as Ruminococcus flavefaciens are regarded essential for the microbial breakdown of cellulose in the rumen. We have investigated the effect of ruminal dosing of R. flavefaciens strain 8/94-32 during realimentation of starved reindeer (males, n = 3). Microbiome function measured as in situ digestion of cellulose and food pellets (percent DMD; dry matter disappearance) decreased after probiotic dosing. Microbial community analyses (>100,000 16S rDNA gene sequences for 27 samples) demonstrated that ruminal dosing influenced the microbiome structure; reflected by increased phylogenetic distances from background samples (unweighted UniFrac analysis) and reduced species diversity and evenness. Despite the inability to detect strain 8/94-32 post-dosing, the relative abundance of its affiliate family Ruminococcaceae remained consistent throughout the trial, whilst a dominant peak in the genus Prevotella and decline in uncharacterized Bacteroidetes (uBacNR) were observed in treatment samples. No clear relationships were observed between the relative abundance of Ruminococcaceae, Prevotella and uBacNR with cellulose DMD; however, Prevotella (negative) and uBacNR (positive) exhibited relationships with pellet DMD. These unexpected effects of ruminal dosing of a cellulolytic bacterium on digestibility are relevant for other studies on rumen manipulation. |
| تدمد: | 1432-184X 0095-3628 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee4def075e83ec406c6d1b1d16c39c1aTest https://doi.org/10.1007/s00248-013-0279-zTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ee4def075e83ec406c6d1b1d16c39c1a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1432184X 00953628 |
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