Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study
| العنوان: | Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study |
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| المؤلفون: | Fang-Hsin Chen, Shu-Hang Ng, Gigin Lin, Mei-Ling Cheng, Cheng-Hsuan Sung, Albert P. Chen, Ying-Chieh Lai, Hung-Yao Ho, Ching-Yi Hsieh, Kuan-Ying Lu |
| المصدر: | Metabolites, Vol 11, Iss 518, p 518 (2021) Metabolites Volume 11 Issue 8 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Endocrinology, Diabetes and Metabolism, cancer metabolism, Microbiology, Biochemistry, Article, dynamic nuclear polarization, chemistry.chemical_compound, Immune system, Western blot, Lactate dehydrogenase, medicine, magnetic resonance imaging, Glycolysis, Molecular Biology, Monocarboxylate transporter, biology, medicine.diagnostic_test, Chemistry, glycolysis, QR1-502, Squamous carcinoma, radiation, Blot, immune system, biology.protein, Cancer research, Flux (metabolism) |
| الوصف: | Alterations in metabolism following radiotherapy affect therapeutic efficacy, although the mechanism underlying such alterations is unclear. A new imaging technique—named dynamic nuclear polarization (DNP) carbon-13 magnetic resonance imaging (MRI)—probes the glycolytic flux in a real-time, dynamic manner. The [1-13C]pyruvate is transported by the monocarboxylate transporter (MCT) into cells and converted into [1-13C]lactate by lactate dehydrogenase (LDH). To capture the early glycolytic alterations in the irradiated cancer and immune cells, we designed a preliminary DNP 13C-MRI study by using hyperpolarized [1-13C]pyruvate to study human FaDu squamous carcinoma cells, HMC3 microglial cells, and THP-1 monocytes before and after irradiation. The pyruvate-to-lactate conversion rate (kPL [Pyr.]) calculated by kinetic modeling was used to evaluate the metabolic alterations. Western blotting was performed to assess the expressions of LDHA, LDHB, MCT1, and MCT4 proteins. Following irradiation, the pyruvate-to-lactate conversion rates on DNP 13C-MRI were significantly decreased in the FaDu and the HMC3 cells but increased in the THP-1 cells. Western blot analysis confirmed the similar trends in LDHA and LDHB expression levels. In conclusion, DNP 13C-MRI non-invasively captured the different glycolytic alterations among cancer and immune systems in response to irradiation, implying its potential for clinical use in the future. |
| وصف الملف: | application/pdf |
| تدمد: | 2218-1989 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a39fba11b1bbfed542f6120dca10bf15Test https://doi.org/10.3390/metabo11080518Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a39fba11b1bbfed542f6120dca10bf15 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22181989 |
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