Overexpression of hepatic 5α-reductase and 11β-hydroxysteroid dehydrogenase type 1 in visceral adipose tissue is associated with hyperinsulinemia in morbidly obese patients
| العنوان: | Overexpression of hepatic 5α-reductase and 11β-hydroxysteroid dehydrogenase type 1 in visceral adipose tissue is associated with hyperinsulinemia in morbidly obese patients |
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| المؤلفون: | Cristian A. Carvajal, Carmen Campino, Carlos E. Fardella, Stefano Macchiavello, Nancy Solís, Mauricio Morales, Camilo Boza, Milan Bozinovic, José Carlos de Miguel Domínguez, Margarita Pizarro, Marco Arrese, Rene Baudrand, Arnoldo Riquelme, Alex Escalona |
| المصدر: | METABOLISM-CLINICAL AND EXPERIMENTAL Artículos CONICYT CONICYT Chile instacron:CONICYT |
| بيانات النشر: | Elsevier BV, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Adult, Blood Glucose, Male, Cholestenone 5 alpha-Reductase, medicine.medical_specialty, Hydrocortisone, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Adipose tissue, Intra-Abdominal Fat, Reductase, Real-Time Polymerase Chain Reaction, Gene Expression Regulation, Enzymologic, Body Mass Index, Endocrinology, Downregulation and upregulation, 11β-hydroxysteroid dehydrogenase type 1, Hyperinsulinism, Internal medicine, 11-beta-Hydroxysteroid Dehydrogenase Type 1, medicine, Hyperinsulinemia, Humans, Insulin, Aspartate Aminotransferases, RNA, Messenger, Cushing Syndrome, Aged, biology, Alanine Transaminase, Middle Aged, medicine.disease, Lipids, Obesity, Morbid, Up-Regulation, C-Reactive Protein, Real-time polymerase chain reaction, Liver, biology.protein, Female, Adiponectin, Cortisone, Biomarkers, hormones, hormone substitutes, and hormone antagonists, medicine.drug |
| الوصف: | 11-β-Hydroxysteroid dehydrogenase type 1 (11β-HSD1) converts cortisone to cortisol, mainly in the liver and visceral adipose tissue (VAT), and has been implicated in several metabolic disorders. The absence of systemic hypercortisolism in central obesity could be due to increased inactivation of cortisol to its tetrahydrometabolites by the hepatic enzymes 5α- and 5β-reductases. Our aim was to assess the expression of the reductases in the liver and of 11β-HSD1 in the liver and VAT in morbidly obese patients and to analyze their association with clinical, anthropometric, and biochemical parameters. Hepatic and VAT samples were obtained during bariatric surgery. 5α- and 5β-reductases, 11β-HSD1, and 18S expression was measured using real-time polymerase chain reaction. Anthropometric and biochemical variables were analyzed. Forty-one patients were recruited (age, 41.8 ± 10.6 years; body mass index, 42.1 ± 6.6 kg/m(2); 71% women). The expression of hepatic 5α- and 5β-reductases was positively correlated (r = +0.53, P = .004), and their expression levels were correlated with hepatic 11β-HSD1 expression (r = +0.61, P < .001 for 5α-reductase and r = +0.50, P < .001 for 5β-reductase). Hepatic 5α-reductase was associated with insulin (r = +0.34, P = .015). Visceral adipose tissue 11β-HSD1 expression was associated with glucose (r = +0.37, P = .025) and insulin (r = +0.54, P = .002). Our results showed that 5α-reductase and VAT 11β-HSD1 expressions were associated with insulinemia. These findings suggest that overexpression of 5α-reductase, through a higher inactivation of cortisol in the liver, could have a protective role in preserving hepatic sensitivity to insulin. The overexpression of liver reductases in obesity could be an adaptive response to an increase in cortisol production by the liver and visceral 11β-HSD1 to avoid systemic hypercortisolism. |
| تدمد: | 0026-0495 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e7680900b78df607941aa21329a80c5Test https://doi.org/10.1016/j.metabol.2011.05.001Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3e7680900b78df607941aa21329a80c5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00260495 |
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