Melanotransferrin is efficiently sorted on the surface of exosomes secreted by melanoma cells
| العنوان: | Melanotransferrin is efficiently sorted on the surface of exosomes secreted by melanoma cells |
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| المؤلفون: | Gaëtan Bellot, Nesrine Aissaoui, Laurent Henry, Marie Morille, Michel Vidal, Anne Bonhoure, Pierre-Emmanuel Stoebner |
| المساهمون: | Dynamique des interactions membranaires normales et pathologiques (DIMNP), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de Biochimie Structurale [Montpellier] (CBS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Melanoma Research Melanoma Research, Lippincott, Williams & Wilkins, 2021, 31, pp.338-351. ⟨10.1097/CMR.0000000000000741⟩ |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Cancer Research, Skin Neoplasms, Dermatology, Exosomes, Mice, Melanoma Biomarker, medicine, Animals, Humans, [CHIM]Chemical Sciences, Melanoma, neoplasms, ComputingMilieux_MISCELLANEOUS, Membrane Glycoproteins, CD63, biology, Chemistry, medicine.disease, Microvesicles, 3. Good health, Oncology, Cutaneous melanoma, Cancer cell, biology.protein, Cancer research, Melanotransferrin, Antibody |
| الوصف: | Cutaneous melanoma is the most lethal type of skin cancer. Early detection is crucial to improve the outcome of melanoma patients. The identification of noninvasive prognostic biomarkers for the follow-up of melanoma patients is still in demand for clinical use. We show here that exosomal melanotransferrin fulfills the biomarker characteristics required to meet this demand. Melanotransferrin is typically overexpressed in melanoma cells compared to other cell types - including cancer cells - and is efficiently sorted and secreted with nanovesicles, or so-called exosomes, due to its membrane-anchoring by a glycosylphosphatidylinositol. Melanotransferrin is exposed on the surface of exosomes and is accessible for antibody recognition. An ELISA was set up to quantify melanotransferrin after immobilization of nanovesicles through the exosomal constituent tetraspanins CD63. Melanotransferrin was detected using a low number of exosomes purified from melanoma cell line cultures, and melanotransferrin detection was abolished by phosphatidylinositol-specific phospholipase C treatment. This exosomal melanotransferrin ELISA was able to discriminate an equal number of assayed exosomes purified from two different melanoma cell lines (A-375 vs. SK-MEL-28). Moreover, plasma samples from patients with melanoma and noncancer disease were assayed using this ELISA and elevated levels of exosomal melanotransferrin were seen in the plasma of patients with melanoma. We propose that exosomal melanotransferrin should be assessed as a potential melanoma biomarker. |
| تدمد: | 0960-8931 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5070fa53f5be90d71502f15bfbf187c5Test https://doi.org/10.1097/cmr.0000000000000741Test |
| رقم الانضمام: | edsair.doi.dedup.....5070fa53f5be90d71502f15bfbf187c5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09608931 |
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