IL-1βand TNFαPromote Monocyte Viability through the Induction of GM-CSF Expression by Rheumatoid Arthritis Synovial Fibroblasts
| العنوان: | IL-1βand TNFαPromote Monocyte Viability through the Induction of GM-CSF Expression by Rheumatoid Arthritis Synovial Fibroblasts |
|---|---|
| المؤلفون: | Régis Brion, Mathias Chatelais, Benoit Le Goff, Dominique Heymann, Marie-Astrid Boutet, Christelle Darrieutort-Laffite, Frédéric Blanchard |
| المصدر: | Mediators of Inflammation Mediators of Inflammation, Vol 2014 (2014) |
| بيانات النشر: | Hindawi Limited, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Macrophage colony-stimulating factor, Article Subject, Cell Survival, CD14, Interleukin-1beta, Immunology, Arthritis, Monocytes, Arthritis, Rheumatoid, lcsh:Pathology, medicine, Humans, Cells, Cultured, Tumor Necrosis Factor-alpha, business.industry, Interleukins, Macrophage Colony-Stimulating Factor, Monocyte, Synovial Membrane, Granulocyte-Macrophage Colony-Stimulating Factor, Cell Biology, Fibroblasts, medicine.disease, medicine.anatomical_structure, Granulocyte macrophage colony-stimulating factor, Culture Media, Conditioned, Rheumatoid arthritis, Tumor necrosis factor alpha, Inflammation Mediators, Synovial membrane, business, lcsh:RB1-214, Research Article, medicine.drug |
| الوصف: | Background. Macrophages and synovial fibroblasts (SF) are two major cells implicated in the pathogenesis of rheumatoid arthritis (RA). SF could be a source of cytokines and growth factors driving macrophages survival and activation. Here, we studied the effect of SF on monocyte viability and phenotype.Methods. SF were isolated from synovial tissue of RA patients and CD14+ cells were isolated from peripheral blood of healthy donors. SF conditioned media were collected after 24 hours of culture with or without stimulation with TNFαor IL-1β. Macrophages polarisation was studied by flow cytometry.Results. Conditioned medium from SF significantly increased monocytes viability by 60% compared to CD14+ cells cultured in medium alone(P<0.001). This effect was enhanced using conditioned media from IL-1βand TNFαstimulated SF. GM-CSF but not M-CSF nor IL34 blocking antibodies was able to significantly decrease monocyte viability by 30% when added to the conditioned media from IL-1βand TNFαstimulated SF(P<0.001). Finally, monocyte cultured in presence of SF conditioned media did not exhibit a specific M1 or M2 phenotype.Conclusion. Overall, rheumatoid arthritis synovial fibroblasts stimulated with proinflammatory cytokines (IL-1βand TNFα) promote monocyte viability via GM-CSF but do not induce a specific macrophage polarization. |
| وصف الملف: | text/xhtml |
| تدمد: | 1466-1861 0962-9351 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::156eefe1b0a7d277da15a6d539740262Test https://doi.org/10.1155/2014/241840Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....156eefe1b0a7d277da15a6d539740262 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14661861 09629351 |
|---|