التفاصيل البيبلوغرافية
| العنوان: |
Long-term tobacco exposure and immunosenescence: Paradoxical effects on T-cells telomere length and telomerase activity. |
| المؤلفون: |
Fernandes, Juliana Ruiz1 (AUTHOR), Pinto, Thalyta Nery Carvalho1 (AUTHOR), Piemonte, Lucas Lopes2 (AUTHOR), Arruda, Liã Barbara3 (AUTHOR), Marques da Silva, Cibele Cristine Berto4 (AUTHOR), F Carvalho, Celso R4 (AUTHOR), Pinto, Regina Maria Carvalho5 (AUTHOR), S Duarte, Alberto J1 (AUTHOR), Benard, Gil1 (AUTHOR) mahong@usp.br |
| المصدر: |
Mechanisms of Ageing & Development. Jul2021, Vol. 197, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
SMOKING, TELOMERES, TELOMERASE, IMMUNOSENESCENCE, OBSTRUCTIVE lung diseases, T cells |
| مستخلص: |
• It is known that telomere length shortening is associated with replicative senescence and can be counteracted by telomerase activity. • However, up regulation of the latter can also favour cell immortalization. • Current smokers without COPD, with a lifespan tobacco exposure had telomerase up regulation and attenuation of telomere shortening. • If causal, the positive effects of tobacco exposure might be mitigated by the chronic pulmonary inflammatory process. Immunosenescence are alterations on immune system that occurs throughout an individual life. The main characteristic of this process is replicative senescence, evaluated by telomere shortening. Several factors implicate on telomere shortening, such as smoking. In this study, we evaluated the influence of smoking and Chronic Obstructive Pulmonary Disease (COPD) on cytokines, telomere length and telomerase activity. Blood samples were collected from subjects aged over 60 years old: Healthy (never smokers), Smokers (smoking for over 30 years) and COPDs (ex-smokers for ≥15 years). A young group was included as control. PBMCs were cultured for assessment of telomerase activity using RT-PCR, and cytokines secretion flow cytometry. CD4+ and CD8+ purified lymphocytes were used to assess telomere length using FlowFISH. We observed that COPD patients have accelerated telomere shortening. Paradoxically, smokers without lung damage showed preserved telomere length, suggesting that tobacco smoking may affect regulatory mechanisms, such as telomerase. Telomerase activity showed diminished activity in COPDs, while Smokers showed increased activity compared to COPDs and Healthy groups. Extracellular environment reflected this unbalance, indicated by an anti-inflammatory profile in Smokers, while COPDs showed an inflammatory prone profile. Further studies focusing on telomeric maintenance may unveil mechanisms that are associated with cancer under long-term smoking. [ABSTRACT FROM AUTHOR] |
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