دورية أكاديمية
Chaperone Hsp47 Drives Malignant Growth and Invasion by Modulating an ECM Gene Network
| العنوان: | Chaperone Hsp47 Drives Malignant Growth and Invasion by Modulating an ECM Gene Network |
|---|---|
| المؤلفون: | Zhu, Jieqing, Xiong, Gaofeng, Fu, Hanjiang, Evers, B. Mark, Zhou, Binhua P., Xu, Ren |
| المصدر: | Markey Cancer Center Faculty Publications |
| بيانات النشر: | UKnowledge |
| سنة النشر: | 2015 |
| المجموعة: | University of Kentucky: UKnowledge |
| مصطلحات موضوعية: | Animals, Cell Proliferation, Cell Transformation, Neoplastic, Extracellular Matrix, Female, Gene Expression Regulation, Gene Regulatory Networks, HEK293 Cells, HSP47 Heat-Shock Proteins, Heterografts, Humans, Mice, SCID, MicroRNAs, Neoplasm Invasiveness, Neoplasm Transplantation, Tumor Cells, Cultured, Oncology |
| الوصف: | The extracellular matrix (ECM) is a determining factor in the tumor microenvironment that restrains or promotes malignant growth. In this report, we show how the molecular chaperone protein Hsp47 functions as a nodal hub in regulating an ECM gene transcription network. A transcription network analysis showed that Hsp47 expression was activated during breast cancer development and progression. Hsp47 silencing reprogrammed human breast cancer cells to form growth-arrested and/or noninvasive structures in 3D cultures, and to limit tumor growth in xenograft assays by reducing deposition of collagen and fibronectin. Coexpression network analysis also showed that levels of microRNA(miR)-29b and -29c were inversely correlated with expression of Hsp47 and ECM network genes in human breast cancer tissues. We found that miR-29 repressed expression of Hsp47 along with multiple ECM network genes. Ectopic expression of miR-29b suppressed malignant phenotypes of breast cancer cells in 3D culture. Clinically, increased expression of Hsp47 and reduced levels of miR-29b and -29c were associated with poor survival outcomes in breast cancer patients. Our results show that Hsp47 is regulated by miR-29 during breast cancer development and progression, and that increased Hsp47 expression promotes cancer progression in part by enhancing deposition of ECM proteins. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | An editorial was written about this article: Xu, R. (2015). MiR-29/Hsp47 in ECM Network. Oncoscience , 2: 843-844. doi: http://dx.doi.org/10.18632/oncoscience.227Test Click here to access this article from UKnowledge.; https://uknowledge.uky.edu/markey_facpub/55Test; https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1057&context=markey_facpubTest |
| الإتاحة: | https://doi.org/10.18632/oncoscience.227Test https://uknowledge.uky.edu/markey_facpub/55Test https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1057&context=markey_facpubTest |
| رقم الانضمام: | edsbas.18B062BE |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |