التفاصيل البيبلوغرافية
| العنوان: |
Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR): Generation of a 5-cM, >5 megabase contig on mouse Chromosome 1. |
| المؤلفون: |
Hunter, K., Ontiveros, S., Watson, M., Stanton, V., Gutierrez, P., Bhat, D., Rochelle, J., Graw, S., Schalling, M., Aburatani, H., Brown, S., Seldin, M., Housman, D. |
| المصدر: |
Mammalian Genome; Oct1994, Vol. 5 Issue 10, p597-607, 11p |
| مستخلص: |
We have developed a new technique for the generation of YAC contigs in the mouse genome that is based on the ability to detect overlapping clones by hybridization of shared IRS-PCR products. As a demonstration of the technique, a 5-cM, >5 megabase contig was developed on the distal half of mouse Chromosome (Chr) 1, spanning the region from Lamb2 to At3. The contig covers roughly 5% of the genetic distance of the chromosome and is comprised of more than 80 clones; 71 probes were assigned physical order on the chromosome, of which 59 were new markers generated in this study. Eight of the new probes were shown to be polymorphic between C3H/HeJ- gld and M. spretus. Three probes were mapped on a [(C3H/HeJ- gld x M. spretus) x C3H/HeJ- gld] interspecific backcross to integrate the physical map with a high-resolution genetic map of the region. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
