المؤلفون: Yongqing Li, Wanshan Ma, Bingyang Zhang, Sumei Lu, Mingju Hao, Peng Gao, Yueyi Zhao, Xiaoxiao Ge, Peijun Wang, Tao Sun, Yanmei Zhang

المصدر: Lipids in Health and Disease
Lipids in Health and Disease, Vol 20, Iss 1, Pp 1-13 (2021)

مصطلحات موضوعية: 0301 basic medicine, Male, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Peroxisome proliferator-activated receptor, 0302 clinical medicine, Endocrinology, Non-alcoholic Fatty Liver Disease, Nonalcoholic fatty liver disease, Nutritional diseases. Deficiency diseases, chemistry.chemical_classification, The PPAR signaling pathway, Fatty liver, Interleukin, Nuclear Proteins, Hepatocyte steatosis, Middle Aged, High-fat diet, Disease Progression, 030211 gastroenterology & hepatology, Tumor necrosis factor alpha, Female, Signal Transduction, Adult, medicine.medical_specialty, RC620-627, Adolescent, Blotting, Western, Biology, Cell Line, 03 medical and health sciences, Young Adult, Downregulation and upregulation, Internal medicine, medicine, Animals, Humans, Transcription factor, Disease Notification, Research, Biochemistry (medical), Twist-Related Protein 1, TWIST family bHLH transcription factor 1 (TWIST1), Glucose Tolerance Test, medicine.disease, Mice, Inbred C57BL, PPAR gamma, Repressor Proteins, 030104 developmental biology, chemistry, TWIST family bHLH transcription factor 2 (TWIST2), Case-Control Studies, Hepatocytes, Steatosis, Peroxisome proliferator activated receptor gamma (PPARγ)

الوصف: Background To investigate the roles of the transcription factors twist family bHLH transcription factor 1 (TWIST1), twist family bHLH transcription factor 2 (TWIST2), and peroxisome proliferator activated receptor gamma (PPARγ) in the progression of nonalcoholic steatohepatitis. Methods The protein levels of TWIST1, TWIST2 and PPARγ were determined in the serum of nonalcoholic fatty liver disease (NAFLD) patients and healthy controls by enzyme-linked immunosorbent assay (ELISA). An in vivo model for fatty liver was established by feeding C57BL/6 J mice a high-fat diet (HFD). An in vitro model of steatosis was established by treating LO-2 cells with oleic acid (OA). RNA sequencing was performed on untreated and OA-treated LO-2 cells followed by TWIST1, TWIST2 and PPARγ gene mRNA levels analysis, Gene Ontology (GO) enrichment and pathway analysis. Results The TWIST2 serum protein levels decreased significantly in all fatty liver groups (P P = 0.0018). The interleukin (IL)-17 and tumor necrosis factor (TNF) signaling pathways were enriched in OA-treated cells. Conclusions The results provide evidence that the TWIST2 and PPAR signaling pathways are important in NAFLD and shed light on a potential mechanism of steatosis.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e85e88081a917ede437b856cc542404dTest
http://europepmc.org/articles/PMC8059034Test

المؤلفون: Jonatan Dereke, Magnus Hillman, Eva O. Melin

المصدر: Lipids in Health and Disease
Lipids in Health and Disease, Vol 19, Iss 1, Pp 1-10 (2020)

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, endocrine system diseases, Adolescent, Clinical chemistry, Cross-sectional study, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Receptor for Advanced Glycation End Products, Clinical nutrition, Gastroenterology, RAGE (receptor), Young Adult, Endocrinology, Glycation, Internal medicine, medicine, Humans, lcsh:RC620-627, Antihypertensive Agents, Inflammation, Type 1 diabetes, business.industry, Research, Biochemistry (medical), S100A12 Protein, nutritional and metabolic diseases, Odds ratio, Extracellular newly identified receptor for advanced glycation end products (EN-RAGE), Middle Aged, medicine.disease, Atherosclerosis, lcsh:Nutritional diseases. Deficiency diseases, Cross-Sectional Studies, Diabetes Mellitus, Type 1, Obesity, Abdominal, Soluble receptor for advanced glycation end products (sRAGE), Female, Hydroxymethylglutaryl-CoA Reductase Inhibitors, business, human activities, Lipidology

الوصف: Background The receptors for advanced glycation end products (RAGE) are increased in atherosclerotic plaques. Soluble (s)RAGE decreases, whereas the extracellular newly identified receptor for advanced glycation end products (EN-RAGE) increases inflammatory responses mediated by RAGE. The aims were to explore whether sRAGE, EN-RAGE and the EN-RAGE/sRAGE ratio, were associated with the use of lipid-lowering drugs (LLD) and/or antihypertensive drugs (AHD) in patients with type 1 diabetes (T1D). Methods Cross-sectional design. T1D patients were consecutively recruited from one diabetes clinic. Blood samples were collected, supplemented with data from electronic health records. sRAGE and EN-RAGE were analysed by enzyme linked immunosorbent assays. An EN-RAGE/sRAGE ratio was calculated. Adjustments were performed with inflammatory and metabolic variables, s-creatinine, depression, smoking, physical inactivity, medication, and cardiovascular complications. Multiple regression analyses were performed. Results In this study 283 T1D patients (men 56%, 18–59 years) were included. One-hundred and thirty LLD users compared to 153 non-users had lower levels of the EN-RAGE/sRAGE ratio (P = 0.009), and 89 AHD users compared to 194 non-users had lower levels of sRAGE (P = 0.031). The use of LLD (inversely) (B coefficient − 0.158, P = 0.033) and the use of AHD (B coefficient 0.187, P = 0.023) were associated with the EN-RAGE/sRAGE ratio. sRAGE (Lg10) (per unit) (adjusted odds ratio (AOR) = 3.5, 95% CI = 1.4–9.1, P = 0.009), EN-RAGE (Lg10) (per unit) (inversely) (AOR 0.4, 95% CI = 0.2–1.0, P = 0.046), age (P P P = 0.001), diabetes duration, triglycerides, s-creatinine, and systolic BP (all P values Conclusions Higher sRAGE levels and lower EN-RAGE levels were linked to the use of LLD, whereas lower sRAGE levels were linked to the use of AHD. No other variables but the use of LLD and the use of AHD were linked to the EN-RAGE/sRAGE ratio. This may be of major importance as sRAGE is an inhibitor and EN-RAGE is a stimulator of inflammatory processes mediated by RAGE.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62626d33a765250960f81c49090ccfe9Test
http://europepmc.org/articles/PMC7557054Test

المؤلفون: Magnus Hillman, Maria Thunander, Ralph Svensson, Hans Thulesius, Eva O. Melin, Mona Landin-Olsson

المصدر: Lipids in Health and Disease
Lipids in Health and Disease, Vol 18, Iss 1, Pp 1-10 (2019)

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Adolescent, Cross-sectional study, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Blood lipids, 030209 endocrinology & metabolism, Clinical nutrition, 030204 cardiovascular system & hematology, Low-grade inflammation, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Internal medicine, medicine, Humans, lcsh:RC620-627, Abdominal obesity, Depression (differential diagnoses), Glycated Hemoglobin, Type 1 diabetes, Serum-lipids, Depression, Research, Biochemistry (medical), Cholesterol, HDL, Odds ratio, Antidepressants, Middle Aged, medicine.disease, Antidepressive Agents, lcsh:Nutritional diseases. Deficiency diseases, Blood pressure, Cross-Sectional Studies, Diabetes Mellitus, Type 1, Obesity, Abdominal, Female, medicine.symptom, Biomarkers

الوصف: Background Depression, metabolic disturbances and inflammation have been linked to cardiovascular disease and mortality. Low levels of high-density lipoprotein cholesterol (HDL-cholesterol), a known marker of cardiovascular risk, have been observed in patients with major depression in psychiatric populations. Our main aim was to explore associations between depression, antidepressants, and metabolic and inflammatory variables in patients with type 1 diabetes (T1D). A secondary aim was to explore variables associated with HDL-cholesterol. Methods Cross-sectional design. T1D patients (n = 292, men 55%, age18–59 years, diabetes duration ≥1 year) were consecutively recruited from one specialist diabetes clinic. Depression was defined as ≥8 points for Hospital Anxiety and Depression Scale-Depression sub scale. Blood samples, anthropometrics, blood pressure, and data regarding medication and life style were collected from electronic health records. Non-parametric tests, multiple logistic and linear regression analyses were performed. Results The depression prevalence was 10 and 8% used antidepressants. Median (q1, q3) HDL-cholesterol (mmol/l) was for the depressed 1.3 (1.2, 1.5) and for the non-depressed 1.6 (1.3, 1.8), p = 0.001. HDL-cholesterol levels (per mmol/l) were negatively associated with depression (Adjusted odds ratio (AOR) 0.2, p = 0.007), and the use of antidepressants was positively associated with depression (AOR 8.1, p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf012c6dd4b0b5fa7fe366534e1bbdc1Test
http://europepmc.org/articles/PMC6421645Test

المؤلفون: Xiao-Yao Li, Qi Yang, Wei-Wei Chen, Guotao Lu, Weiqin Li, Na Pu, Zhihui Tong, Jian-Min Chen, Xiao-Lei Shi

المصدر: Lipids in Health and Disease
Lipids in Health and Disease, Vol 18, Iss 1, Pp 1-5 (2019)

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Heterozygote, Endocrinology, Diabetes and Metabolism, media_common.quotation_subject, Clinical Biochemistry, Nonsense, Nonsense mutation, 030209 endocrinology & metabolism, 030204 cardiovascular system & hematology, 03 medical and health sciences, symbols.namesake, Exon, 0302 clinical medicine, Endocrinology, Internal medicine, medicine, Humans, Lipase maturation factor 1, Genetic Predisposition to Disease, Gene, lcsh:RC620-627, Life Style, media_common, Sanger sequencing, Hypertriglyceridemia, business.industry, Research, Biochemistry (medical), Smoking, GPIHBP1, Membrane Proteins, medicine.disease, Acute pancreatitis, Obesity, Morbid, lcsh:Nutritional diseases. Deficiency diseases, Pancreatitis, Codon, Nonsense, Mutation, symbols, business

الوصف: Background Hypertriglyceridemia (HTG) is one of the most common etiologies of acute pancreatitis (AP). Variants in five genes involved in the regulation of plasma lipid metabolism, namely LPL, APOA5, APOC2, GPIHBP1 and LMF1, have been frequently reported to cause or predispose to HTG. Methods A Han Chinese patient with HTG-induced AP was assessed for genetic variants by Sanger sequencing of the entire coding and flanking sequences of the above five genes. Results The patient was a 32-year-old man with severe obesity (Body Mass Index = 35) and heavy smoking (ten cigarettes per day for more than ten years). At the onset of AP, his serum triglyceride concentration was elevated to 1450.52 mg/dL. We sequenced the entire coding and flanking sequences of the LPL, APOC2, APOA5, GBIHBP1 and LMF1 genes in the patient. We found no putative deleterious variants, with the exception of a novel and heterozygous nonsense variant, c.1024C > T (p.Arg342*; rs776584760), in exon 7 of the LMF1 gene. Conclusions This is the first time that a heterozygous LMF1 nonsense variant was found in a HTG-AP patient with severe obesity and heavy smoking, highlighting an important interplay between genetic and lifestyle factors in the etiology of HTG. Electronic supplementary material The online version of this article (10.1186/s12944-019-1012-9) contains supplementary material, which is available to authorized users.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73b5ccd419d40c0d066c0b7d614c7ed1Test
http://europepmc.org/articles/PMC6421687Test

المؤلفون: Liqiu Yan, Zhe Li, Zheng Lian, Xufen Cao, Fengfeng Lv, Yunfei Wang, Saitian Zeng, Yanshen Li, Jiawang Wang

المصدر: Lipids in Health and Disease

مصطلحات موضوعية: 0301 basic medicine, Pathology, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Disease, Coronary Artery Disease, 030204 cardiovascular system & hematology, Coronary Angiography, Gastroenterology, p38 Mitogen-Activated Protein Kinases, Coronary artery disease, chemistry.chemical_compound, 0302 clinical medicine, Endocrinology, Risk Factors, Family history, p38MAPK-1, Coronary lesion characteristics, Middle Aged, Prognosis, Postmenopause, Female, medicine.symptom, Lipidology, Signal Transduction, Adult, medicine.medical_specialty, HO-1, Clinical nutrition, Lesion, 03 medical and health sciences, Internal medicine, Diabetes mellitus, medicine, Humans, Aged, Biochemistry, medical, business.industry, Cholesterol, Research, Biochemistry (medical), HIF-1, medicine.disease, Hypoxia-Inducible Factor 1, alpha Subunit, 030104 developmental biology, chemistry, Gene Expression Regulation, Premenopause, Perimenopausal CAD, business, Heme Oxygenase-1

الوصف: Background The present study was intended to explore whether three proteins within MAPK signaling pathway (i.e. p38MAPK-1, HIF-1 and HO-1) were correlated with peri-menopausal women’s coronary lesion features and prognosis. Methods Altogether 1449 peri-menopausal women were divided into non-coronary artery disease (CAD) group (n = 860) and CAD group (n = 589), including 167 pre-menopausal CAD populations and 422 post-menopausal CAD populations. General information about CAD risk parameters were gathered, including age, family history of CAD or hypertension or diabetes mellitus, bilirubin, cholesterol, triglyceride, high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) and so on. Coronary angiography results were judged, and CAD score was calculated with application of Genisin scoring method. Besides, detection of MAPK-1 levels was implemented with Strept Avidin-Biotin Complex (SABC) method, while HIF-1 and HO-1 expressions in the serum were determined utilizing ELISA detection kit. Correlations among protein expressions, characteristics of coronary lesions and prognosis of CAD populations were finally evaluated. Results Hypertension, hyperlipoidemia, diabetes and smoking history were more prevalent among postmenopausal CAD women than premenopausal CAD women (P premenopausal CAD women > non-CAD women. Moreover, p38MAPK-1, HIF-1 and HO-1 expressions were up-regulated with increasing number of vessel lesions and severity of coronary lesions among peri-menopausal women. Besides, among both pre-menopausal and post-menopausal CAD groups, positive correlations could be observed between MAPK-1 and TG (r s = 0.271; r s = 0.476), between HIF-1α and LDL-C (r s = 0.077; r s = 0.470), as well as between HO-1 and CysC (r s = 0.492; r s = 0.190) or hs-CRP (r s = 0.569; r s = 0.542) (all P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0778330dd6a3096e71d8c72475a3239cTest
http://europepmc.org/articles/PMC5100280Test

المؤلفون: Georgios Vasilopoulos, Dimitrios Degiannis, Agathi Karakosta, Christina Marvaki, Apostolia Marvaki, Genovefa Kolovou, Vana Kolovou, Sophie Mavrogeni, Antonia Kalogiani

المصدر: Lipids in Health and Disease
Lipids in Health and Disease, Vol 11, Iss 1, p 62 (2012)

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Blood lipids, Nurses, Single-nucleotide polymorphism, Polymorphism, Single Nucleotide, Young Adult, Endocrinology, Gene Frequency, Internal medicine, Genotype, medicine, ABCA1 Gene, Humans, lcsh:RC620-627, Allele frequency, Genetic Association Studies, Genetics, Biochemistry, medical, Electrophoresis, Agar Gel, Sex Characteristics, biology, medicine.diagnostic_test, Greece, Research, Biochemistry (medical), Lipids, lcsh:Nutritional diseases. Deficiency diseases, biology.protein, Population study, Apolipoprotein A1, lipids (amino acids, peptides, and proteins), ATP-Binding Cassette Transporters, Female, Lipid profile, ATP Binding Cassette Transporter 1

الوصف: Objective One of the important proteins involved in lipid metabolism is the ATP-binding cassette transporter A1 (ABCA1) encoding by ABCA1 gene. In this study we evaluated the single nucleotide polymorphisms (SNPs) of ABCA1 gene. We analyzed SNPs in chromosome 9 such as rs2230806 (R219K) in the position 107620867, rs2230808 (R1587K) in the position 106602625 and rs4149313 (I883M) in the position 106626574 according to gender and lipid profile of Greek nurses. Methods The study population consisted of 447 (87 men) unrelated nurses who were genotyped for ABCA1 gene polymorphisms. Additionally, lipid profile [total cholesterol, triglycerides, high density lipoprotein cholesterol, low density lipoprotein cholesterol (LDL-C) and apolipoprotein A1] was evaluated. Results The distribution of all three studied ABCA1 gene polymorphisms did not differ according to gender. However, only R219K genotype distribution bared borderline statistical significance (p = 0.08) between the two studied groups. Moreover, allele frequencies of R219K, R1587K and I88M polymorphisms did not differ according to gender. In general, blood lipid levels did not seem to vary according to ABCA1 gene polymorphisms, when testing all subjects or when testing only men or only women. However, a significant difference of LDL-C distribution was detected in all subjects according to R1587K genotype, indicating lower LDL-C levels with KK polymorphism (p = 0.0025). The above difference was solely detected on female population (p = 0.0053). Conclusions The ABCA1 gene polymorphisms frequency, distribution and lipid profile did not differ according to gender. However, in the female population the KK genotype of R1587K gene indicated lower LDL-C levels. Further studies, involving a higher number of individuals, are required to clarify genes and gender contribution.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e52c5890ed239a8a816b20569f70cf25Test
http://europepmc.org/articles/PMC3391977Test