دورية أكاديمية
The Cell Protective Effect of Adenine on Hypoxia–Reoxygenation Injury through PPAR Delta Activation
| العنوان: | The Cell Protective Effect of Adenine on Hypoxia–Reoxygenation Injury through PPAR Delta Activation |
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| المؤلفون: | Jyh-Gang Leu, Chien-Mei Wang, Chao-Yi Chen, Yi-Feng Yang, Chin-Yu Shih, Jiun-Tsai Lin, Han-Min Chen, Yao-Jen Liang |
| المصدر: | Life, Vol 11, Iss 12, p 1408 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Science |
| مصطلحات موضوعية: | adenine, ischemia-reperfusion, myocardial infarction, antioxidation, cell cycle, Science |
| الوصف: | Ischemia followed by blood supply reperfusion in cardiomyocytes leads to an overproduction of free radicals and a rapid decrease of adenosine triphosphate concentration. The cardioprotective effect of a potential drug, adenine, was evaluated using H9c2 rat cardiomyoblasts. After hypoxia–reoxygenation (HR) treatment consisting of hypoxia for 21 h followed by reoxygenation for 6 h, it was revealed that pretreatment with 200 µM adenine for 2 h effectively prevented HR-induced cell death. Adenine also significantly decreased the production of reactive oxygen species and reduced cell apoptosis after HR injury. The antioxidant effect of adenine was also revealed in this study. Adenine pretreatment significantly reduced the expression of activating transcription factor 4 (ATF4) and glucose-regulated protein 78 (GRP78) proteins, and protein disulfide isomerase induced a protective effect on mitochondria after HR stimulation. Intracellular adenosine monophosphate-activated protein kinase, peroxisome proliferator-activated receptor delta (PPARδ), and perilipin levels were increased by adenine after HR stimulation. Adenine had a protective effect in HR-damaged H9c2 cells. It may be used in multiple preventive medicines in the future. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 11121408 2075-1729 |
| العلاقة: | https://www.mdpi.com/2075-1729/11/12/1408Test; https://doaj.org/toc/2075-1729Test |
| DOI: | 10.3390/life11121408 |
| الوصول الحر: | https://doaj.org/article/a64b945b89194a6c8f90398f6babcb5cTest |
| رقم الانضمام: | edsdoj.64b945b89194a6c8f90398f6babcb5c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 11121408 20751729 |
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| DOI: | 10.3390/life11121408 |