Towards high throughput production of artificial egg oocytes using microfluidics
| العنوان: | Towards high throughput production of artificial egg oocytes using microfluidics |
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| المؤلفون: | Angela Jimenez, Matthieu Roché, Zoher Gueroui, Laurent Courbin, Pascal Panizza, Mathieu Pinot |
| المساهمون: | Institut de Physique de Rennes (IPR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Groupe matière condensée et matériaux (GMCM), Association pour la Recherche sur le Cancer (ARC--4474), CNRS PIR interface, Ligue Nationale Contre le Cancer (LNCC, 2009), ANR-08-NANO-0050,Biomodulator,biomodulator(2008), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), ANR-08-NANO-0050,Biomodulator,Fluorescent Magnetic Nanopaticles as modulators of signaling pathways(2008) |
| المصدر: | Lab on a Chip Lab on a Chip, Royal Society of Chemistry, 2011, 11 (3), pp.429-34. ⟨10.1039/c0lc00046a⟩ Lab on a Chip, 2011, 11 (3), pp.429-34. ⟨10.1039/c0lc00046a⟩ |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Microfluidics, Biomedical Engineering, Environment controlled, MESH: Microscopy, Fluorescence, Bioengineering, 02 engineering and technology, Biology, complex mixtures, Biochemistry, Microtubules, MESH: Oocytes, MESH: Linear Models, MESH: Microfluidics, 03 medical and health sciences, Xenopus laevis, Biopolymers, MESH: Xenopus laevis, Microtubule, MESH: Dimethylpolysiloxanes, Fluorescence microscope, Animals, MESH: Animals, Dimethylpolysiloxanes, Cytoskeleton, Throughput (business), Eukaryotic cell, 030304 developmental biology, 0303 health sciences, MESH: Microtubules, Drug discovery, technology, industry, and agriculture, General Chemistry, Microfluidic Analytical Techniques, 021001 nanoscience & nanotechnology, Cell biology, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, MESH: Biopolymers, Actin Cytoskeleton, Nylons, Microscopy, Fluorescence, MESH: Microfluidic Analytical Techniques, Linear Models, Oocytes, MESH: Nylons, MESH: Actin Cytoskeleton, 0210 nano-technology |
| الوصف: | International audience; The production of micron-size droplets using microfluidic tools offers new opportunities to carry out biological assays in a controlled environment. We apply these strategies by using a flow-focusing microfluidic device to encapsulate Xenopus egg extracts, a biological system recapitulating key events of eukaryotic cell functions in vitro. We present a method to generate monodisperse egg extract-in-oil droplets and use high-speed imaging to characterize the droplet pinch-off dynamics leading to the production of trains of droplets. We use fluorescence microscopy to show that our method does not affect the biological activity of the encapsulated egg extract by observing the self-organization of microtubules and actin filaments, two main biopolymers of the cell cytoskeleton, encapsulated in the produced droplets. We anticipate that this assay might be useful for quantitative studies of biological systems in a confined environment as well as high throughput screenings for drug discovery. |
| تدمد: | 1473-0189 1473-0197 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f185418a593f58f2d64aa8003e42109Test https://pubmed.ncbi.nlm.nih.gov/21072407Test |
| رقم الانضمام: | edsair.doi.dedup.....7f185418a593f58f2d64aa8003e42109 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14730189 14730197 |
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