التفاصيل البيبلوغرافية
| العنوان: |
DNA methylation analysis on a droplet-in-oil PCR array. |
| المؤلفون: |
Yi Zhang, Vasudev Bailey, Christopher M. Puleo, Hariharan Easwaran, Elizabeth Griffiths, James G. Herman, Stephen B. Baylin, Tza-Huei Wang |
| المصدر: |
Lab on a Chip; Apr2009, Vol. 9 Issue 8, p1059-1064, 6p |
| مصطلحات موضوعية: |
METHYLATION, DNA microarrays, POLYMERASE chain reaction, MICROFLUIDICS, DROPLETS |
| مستخلص: |
We performed on-chip DNA methylation analysis using methylation-specific PCR (MSP) within an arrayed micro droplet-in-oil platform that is designed for more practical application of microfluidic droplet technologies in clinical applications. Unique features of this ready-to-use device include arrayed primers that are pre-deposited into open micro-reaction chambers and use of the oil phase as a companion fluid for both sample actuation and compartmentalization. These technical advantages allow for infusion of minute amounts of sample for arrayed MSP analysis, without the added complexities inherent in microfluidic droplet-based studies. Ease of use of this micro device is exemplified by analysis of two tumor suppressor promoters, p15and TMS1using an on-chip methylation assay. These results were consistent with standard MSP protocols, yet the simplicity of the droplet-in-oil microfluidic PCR platform provides an easy and efficient tool for DNA methylation analysis in a large-scale arrayed manner. [ABSTRACT FROM AUTHOR] |
|
Copyright of Lab on a Chip is the property of Royal Society of Chemistry and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
