P-glycoprotein-deficient mice have proximal tubule dysfunction but are protected against ischemic renal injury
| العنوان: | P-glycoprotein-deficient mice have proximal tubule dysfunction but are protected against ischemic renal injury |
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| المؤلفون: | Leo A. J. Kluijtmans, J W A van der Hoorn, Frans G. M. Russel, C Kramers, Rosalinde Masereeuw, Elena Levtchenko, Martijn J. Wilmer, Miriam Huls, Henry B.P.M. Dijkman |
| المساهمون: | TNO Kwaliteit van Leven |
| المصدر: | Kidney International. Supplement, 72, 1233-41 Kidney International, 10, 72, 1233-1241 Kidney International. Supplement, 72, 10, pp. 1233-41 |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Male, amino acid urine level, calcium excretion, glomerulus filtration rate, kidney dysfunction, Membrane transport and intracellular motility [NCMLS 5], Fluorescent Antibody Technique, Vascular medicine and diabetes [UMCN 2.2], glycoprotein P, ischemia-reperfusion, Kidney Tubules, Proximal, chemistry.chemical_compound, Mice, Ischemia, creatinine clearance, oxidative stress, glucose, glucosuria, plasma clearance, Amino Acids, sodium, Renal disorder [IGMD 9], Mice, Knockout, Kidney, Ischemia-reperfusion, creatinine, protein function, Acute Kidney Injury, Immunohistochemistry, Mitochondria, Proteinuria, medicine.anatomical_structure, kidney tubule necrosis, priority journal, lithium, Nephrology, Knockout mouse, kidney injury, ABC transporter, amino acid, Glomerular Filtration Rate, medicine.medical_specialty, adenosine triphosphate, kidney cortex, Diuresis, urinary excretion, Mice, Inbred Strains, Synthetic Organic Chemistry, Drug transporter, Biology, animal tissue, Renal Circulation, Genomic disorders and inherited multi-system disorders [IGMD 3], kidney proximal tubule, Glycosuria, Internal medicine, medicine, Kidney dysfunction, Animals, controlled study, ATP Binding Cassette Transporter, Subfamily B, Member 1, protein expression, kidney blood flow, Creatinine, calcium, Renal circulation, electron microscopy, Renal ischemia, animal model, Sodium, P-Glycoprotein, kidney ischemia, Kidney Failure, Acute, Apical membrane, drug transporter, Renal disorders [UMCN 5.4], Endocrinology, chemistry, Evaluation of complex medical interventions [NCEBP 2], Renal blood flow, Calcium, sodium excretion, protein, knockout mouse, upregulation |
| الوصف: | The multidrug resistance gene 1 product, P-glycoprotein (P-gp), is expressed in several excretory organs, including the apical membrane of proximal tubules. After inducing acute renal failure, P-gp expression is upregulated and this might be a protective function by pumping out toxicants and harmful products of oxidative stress. We characterized renal function of P-gp knockout mice and studied its consequences in renal ischemic damage. Compared with wild-type mice, knockout mice have a lower glomerular filtration rate and renal plasma flow. An augmented urinary excretion of sodium, numerous amino acids, calcium, glucose, and low molecular weight proteins was observed along with an increased diuresis. A higher lithium plasma clearance in the knockout mice suggested proximal tubular dysfunction. Electron microscopy showed mitochondrial abnormalities in proximal tubular cells that could account for decreased adenosine triphosphate levels in the cortex. After inducing ischemia, wild-type mice showed a decrease in creatinine clearance and severe proximal tubular necrosis. In contrast, knockout mice had no signs of tubular damage. Our data indicate that P-gp knockout mice have impaired renal function but are protected against ischemic renal injury. © 2007 International Society of Nephrology. Chemicals/CAS: adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; amino acid, 65072-01-7; calcium, 7440-70-2; creatinine, 19230-81-0, 60-27-5; glucose, 50-99-7, 84778-64-3; lithium, 7439-93-2; protein, 67254-75-5; sodium, 7440-23-5; Amino Acids; Calcium, 7440-70-2; P-Glycoprotein; Sodium, 7440-23-5 |
| وصف الملف: | application/pdf |
| تدمد: | 2157-1724 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bfdff93c99ba4280d54b6461a703770fTest https://hdl.handle.net/2066/36285Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....bfdff93c99ba4280d54b6461a703770f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21571724 |
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