Acute sodium overload produces renal tubulointerstitial inflammation in normal rats
| العنوان: | Acute sodium overload produces renal tubulointerstitial inflammation in normal rats |
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| المؤلفون: | Jorge E. Toblli, O. Canessa, Marcela Pandolfo, Gabriel Cao, A. Kuprewicz, S. L. Della Penna, María Inés Rosón, Susana Cavallero, Belisario E. Fernández, Susana Gorzalczany |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| بيانات النشر: | Elsevier BV, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Male, transforming growth factor, Ciencias de la Salud, Blood Pressure, Rats, Sprague-Dawley, SODIUM OVERLOAD, Transforming Growth Factor beta, Fibrosis, Chemokine CCL5, Angiotensin II, NF-kappa B, Immunohistochemistry, Otras Ciencias de la Salud, Kidney Tubules, Nephrology, purl.org/becyt/ford/3 [https], RENAL TUBULOINTERSTITIAL INFLAMMATION, sodium overload, medicine.medical_specialty, Mean arterial pressure, CIENCIAS MÉDICAS Y DE LA SALUD, Sodium, Cell Respiration, chemistry.chemical_element, Renal function, SMOOTH MUSCLE ACTIN, Transforming Growth Factor beta1, Excretion, purl.org/becyt/ford/3.3 [https], RANTES, renal tubulointerstitial inflammation, Internal medicine, medicine, Animals, Inflammation, Dose-Response Relationship, Drug, Biological Transport, medicine.disease, Actins, Rats, Endocrinology, TRANSFORMING GROWTH FACTOR, chemistry, Regional Blood Flow, Renal blood flow, smooth muscle actin, Nephritis, Interstitial, Transforming growth factor |
| الوصف: | The aim of the present study was to determine whether acute sodium overload could trigger an inflammatory reaction in the tubulointerstitial (TI) compartment in normal rats. Four groups of Sprague-Dawley rats received increasing NaCl concentrations by intravenous infusion. Control (C): Na + 0.15M; G1: Na+ 0.5M; G2: Na+ 1.0M; and G3: Na+ 1.5M. Creatinine clearance, mean arterial pressure (MAP), renal blood flow (RBF), and sodium fractional excretion were determined. Transforming growth factor β1 (TGF-β1), α-smooth muscle actin (α-SMA), RANTES, transcription factor nuclear factor-kappa B (NF-κB), and angiotensin II (ANG II) were evaluated in kidneys by immunohistochemistry. Animals with NaCl overload showed normal glomerular function without MAP and RBF modifications and exhibited a concentration-dependent natriuretic response. Plasmatic sodium increased in G2 (P < 0.01) and G3 (P < 0.001). Light microscopy did not show renal morphological damage. Immunohistochemistry revealed an increased number of ANG II-positive tubular cells in G2 and G3, and positive immunostaining for NF-κB only in G3 (P < 0.01). Increased staining of α-SMA in the interstitium (P < 0.01), TGF-β1 in tubular cells (P < 0.01), and a significant percentage (P < 0.01) of positive immunostaining for RANTES in tubular epithelium and in glomerular and peritubular endothelium were detected in G3>G2>C group. These results suggest that an acute sodium overload is able 'per se' to initiate TI endothelial inflammatory reaction (glomerular and peritubular) and incipient fibrosis in normal rats, independently of hemodynamic modifications. Furthermore, these findings are consistent with the possibility that activation of NF-κB and local ANG II may be involved in the pathway of this inflammatory process. Fil: Roson, Maria Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cavallero, Carmen Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Della Penna, Silvana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Cao, Gabriel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Alemán; Argentina Fil: Gorzalczany, Susana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Pandolfo, Marcela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Kuprewicz, A.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Canessa, O.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Toblli, Jorge Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Alemán; Argentina Fil: Fernandez, Belisario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina |
| وصف الملف: | application/pdf |
| تدمد: | 0085-2538 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e073e365a9076ede2fd91477c687d445Test https://doi.org/10.1038/sj.ki.5001831Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e073e365a9076ede2fd91477c687d445 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00852538 |
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