Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein
| العنوان: | Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein |
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| المؤلفون: | Guan-Ling Lin, Der-Shan Sun, Jing-Hua Dong, Yu-Cheng Hsiao, Po-Kong Chen, Chih-Hsien Liao, Hsin-Hou Chang, Chun-Jen Wang |
| المصدر: | Journal of Virological Methods |
| بيانات النشر: | Elsevier BV, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | S, spike, Middle East respiratory syndrome coronavirus, viruses, Virus Attachment, Spike protein, Biology, ACE2, angiotensin-converting enzyme 2, medicine.disease_cause, Antiviral Agents, Article, Cell Line, Microbiology, Binding motif, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Virology, Cell Adhesion, medicine, Animals, Humans, Binding site, Cell adhesion, SARS-CoV, severe acute respiratory syndrome-coronavirus, chemistry.chemical_classification, Binding Sites, fungi, RBM, receptor-binding motif, food and beverages, RBD, receptor-binding domain, virus diseases, Spike Protein, SARS-CoV, Cell Adhesion Inhibition, Amino acid, Severe acute respiratory syndrome-related coronavirus, chemistry, Spike Glycoprotein, Coronavirus, Peptide vaccine, Vero cell, Vero E6 cells, Peptides, Protein Binding |
| الوصف: | Highlights • Cell adhesion assay is a simple method to identify the receptor-binding motif of the SARS-CoV spike. • Synthetic peptides can be used to narrow the receptor-binding motif. • The 436–445 amino acids are the minimum receptor-binding motif of the SARS-CoV spike. • Cell adhesion assay can be developed as a high throughput system by using a 96-well MTT assay. Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and safe method to investigate a therapeutic approach against these pathogens is required. In this study, a simple, quick, and safe cell adhesion inhibition assay was developed to determine the potential cellular binding site on the SARS-CoV spike protein. Various synthetic peptides covering the potential binding site helped to minimize further the binding motif to 10–25 residues. Following analyses, 2 peptides spanning the 436–445 and 437–461 amino acids of the spike protein were identified as peptide inhibitor or peptide vaccine candidates against SARS-CoV. |
| تدمد: | 0166-0934 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77cab9cdde8a58dba877279ecc6b6828Test https://doi.org/10.1016/j.jviromet.2014.01.022Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....77cab9cdde8a58dba877279ecc6b6828 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01660934 |
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