Dog Steroidogenic Factor-1: Molecular cloning and analysis of epigenetic regulation
| العنوان: | Dog Steroidogenic Factor-1: Molecular cloning and analysis of epigenetic regulation |
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| المؤلفون: | Asato Sekiya, Yoshikazu Arai, Koichiro Nishino, Ken Takasawa, Shidow Torisu |
| المصدر: | The Journal of Veterinary Medical Science |
| بيانات النشر: | Japanese Society of Veterinary Science, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, molecular cloning, Steroidogenic factor 1, DNA, Complementary, 040301 veterinary sciences, Sequence alignment, Biology, Steroidogenic Factor 1, Biochemistry, Epigenesis, Genetic, 0403 veterinary science, 03 medical and health sciences, Exon, Dogs, Complementary DNA, Adrenal Glands, Testis, Animals, Coding region, Amino Acid Sequence, Cloning, Molecular, Gene, 030304 developmental biology, Genetics, 0303 health sciences, DNA methylation, promoter, Full Paper, General Veterinary, Ovary, Sequence Analysis, DNA, 04 agricultural and veterinary sciences, Adipose Tissue, Gene Expression Regulation, CpG site, dog, Female, Sequence Alignment |
| الوصف: | Steroidogenic factor 1 (SF-1) is a nuclear receptor that is important in steroid hormone production, and adrenal and gonad development. The SF-1 gene is highly conserved among most vertebrates. However, dog SF-1 registered in public databases, such as CanFam3.1, lacks the 5’ end compared to other mammals including mouse, human, bovine, and cat. Whether this defect is due to species differences or database error is unclear. Here, we determined the full-length dog SF-1 cDNA sequence and identified the missing 5’ end sequence in the databases. The coding region of the dog SF-1 gene has 1,386 base pairs, and the protein has 461 amino acid residues. Sequence alignment analysis among vertebrates revealed that the 5’ end sequence of dog SF-1 cDNA is highly conserved compared to other vertebrates. The genomic position of the first exon was determined, and its promoter region sequence was analyzed. The DNA methylation state at the basal promoter and the expression of dog SF-1 in steroidogenic tissues and non-steroidogenic cells were examined. CpG sites at the basal promoter displayed methylation kinetics inversely correlated with gene expression. The promoter was hypomethylated and hypermethylated in SF-1 expressing and non-SF-1 expressing tissues, respectively. In conclusion, we identified the true full sequence of dog SF-1 cDNA and determined the genome sequence around the first exon. The gene is under the control of epigenetic regulation, such as DNA methylation, at the promoter. |
| تدمد: | 1347-7439 0916-7250 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c0fe841f72bbe7e6778ba800794cb94Test https://doi.org/10.1292/jvms.20-0050Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....6c0fe841f72bbe7e6778ba800794cb94 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13477439 09167250 |
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